Electrical imaging of neuronal activity by multi-transistor-array (MTA) recording at 7.8 μm resolution
Electrical imaging of neuronal activity by multi-transistor-array (MTA) recording at 7.8 μm resolution
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DOI：
10.1007/s00339-004-2991-5
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发表时间：
2004-11-01
影响因子：
2.7
通讯作者：
Fromherz, P
中科院分区：
文献类型：
--
作者：
Lambacher, A;Jenkner, M;Fromherz, P
我们报告了一种新的方法，用于神经元活动的二维电映射，使用1 mm（2）阵列的16384传感器场效应晶体管制造的扩展CMOS（互补金属氧化物硅）技术。从神经元到芯片的接触是由芯片表面上的绝缘氧化物形成的，该绝缘氧化物提供传感器晶体管的纯电容驱动。作为多晶体管阵列（MTA）记录的测试系统，我们使用培养的神经元从池塘蜗牛。我们提出了一个单独的神经元和一个小的神经元网络的分辨率为7.8妈妈的电子地图。MTA记录为神经生物学和生物传感器学研究提供了一种对培养的神经细胞和脑切片的网络动力学进行成像的工具。
We report on a novel method for two-dimensional electrical mapping of neuronal activity using a 1 mm(2) array of 16384 sensor field-effect transistors fabricated by an extended CMOS (complementary metal oxide silicon) technology. The contact from neurons to chip is made by an insulating oxide on the chip surface that provides a purely capacitive drive of the sensor transistors. As a test system for multi-transistor-array (MTA) recording we use cultured neurons from the pond snail. We present electrical maps of an individual neuron and of a small neuronal network at a resolution of 7.8 mum. MTA recording provides a tool for imaging the network dynamics of cultured nerve cells and brain slices for studies in neurobiology and biosensorics.