Comparison of haematology, coagulation and clinical chemistry parameters in blood samples from the sublingual vein and vena cava in Sprague-Dawley rats
Comparison of haematology, coagulation and clinical chemistry parameters in blood samples from the sublingual vein and vena cava in Sprague-Dawley rats
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DOI：
10.1258/la.2010.009049
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发表时间：
2010-10-01
影响因子：
2.4
通讯作者：
Blaich, G.
中科院分区：
文献类型：
--
作者：
Seibell, J.;Bodie, K.;Blaich, G.
临床病理参数(血液学、临床化学和凝血)的研究是药物安全性临床前评价的重要组成部分。然而，所采用的血液采样方法应避免或最大限度地减少实验动物的压力和伤害。在本研究中，我们比较了在尸检前立即从下腔静脉(VC)采集的血液标本和在尸检前从舌下静脉(VS)采集的血液样本的临床病理结果，以确定采样方法是否对临床病理参数有影响。将46只12周龄雄性SD大鼠随机分为两组(VC组和VS组，每组23只)。所有大鼠在取材前用异氟醚麻醉。VC组从下腔静脉取血。对于VS采样，轻轻地拔出舌头并刺穿VS。比较两组患者的血液学、凝血及临床生化指标。建立了13个参数的等价性，如平均红细胞体积、白细胞和钙。没有发现其余26个参数的等值，尽管与历史数据和正常范围相比，它们被认为是相似的。最显著的发现是VC组的活化凝血酶原时间(16.6+/-0.89 S)比VS组(23.8+/-1.58 S)少30.3%。综上所述，在临床前药物安全性研究中，在尸检前从下腔静脉采血似乎是一种合适和可靠的终端采血方法，可以减少对实验室大鼠的压力和伤害。
The investigation of clinical pathology parameters (haematology, clinical chemistry and coagulation) is an important part of the preclinical evaluation of drug safety. However, the blood sampling method employed should avoid or minimize stress and injury in laboratory animals. In the present study, we compared the clinical pathology results from blood samples collected terminally from the vena cava (VC) immediately before necropsy with samples taken from the sublingual vein (VS) also prior to necropsy in order to determine whether the sampling method has an influence on clinical pathology parameters. Forty-six 12-week-old male Sprague-Dawley rats were assigned to two groups (VC or VS; n = 23 each). All rats were anaesthetized with isoflurane prior to sampling. In the VC group, blood was withdrawn from the inferior VC. For VS sampling, the tongue was gently pulled out and the VS was punctured. The haematology, coagulation and clinical chemistry parameters were compared. Equivalence was established for 13 parameters, such as mean corpuscular volume, white blood cells and calcium. No equivalence was found for the remaining 26 parameters, although they were considered to be similar when compared with the historical data and normal ranges. The most conspicuous finding was that activated prothrombin time was 30.3% less in blood taken from the VC (16.6 +/- 0.89 s) than that in the VS samples (23.8 +/- 1.58 s). Summing up, blood sampling from the inferior VC prior to necropsy appears to be a suitable and reliable method for terminal blood sampling that reduces stress and injury to laboratory rats in preclinical drug safety studies.