Three-dimensional scaffold of type II collagen promote the differentiation of adipose-derived stem cells into a nucleus pulposus-like phenotype
Three-dimensional scaffold of type II collagen promote the differentiation of adipose-derived stem cells into a nucleus pulposus-like phenotype
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II型胶原三维支架促进脂肪干细胞分化为髓核样表型
DOI：
10.1002/jbm.a.35701
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发表时间：
2016
影响因子：
4.9
通讯作者：
Chen Qixin
中科院分区：
文献类型：
--
作者：
Zhou Xiaopeng;Tao Yiqing;Wang Jingkai;Liu Dongyu;Liang Chengzhen;Li Hao;Chen Qixin
据报道，II型胶原蛋白具有引导脂肪源性干细胞（ADSC）向髓核（NP）样表型分化的能力。因此本研究旨在利用N，N-（3-二甲氨基丙基）-N′-乙基碳二亚胺和N-羟基琥珀酰亚胺（EDAC/NHS）构建三维胶原支架，以提高ADSC向NP样细胞分化的效率。测量物理性质，如孔隙率、生物降解性和微观结构，以及生物学特性，如细胞毒性、细胞增殖以及相关基因和蛋白质的表达，以评估不同支架的功效。与其他组相比，与EDAC/NHS交联的胶原支架表现出更高的生物稳定性，更好的空间结构，以及更高的功能标记物如聚集蛋白聚糖，SOX 9和COL 2的基因和蛋白表达。基于结果，与EDAC/NHS交联的冻干II型胶原形成了最好的3D支架，用于诱导ADSC增殖和向NP样表型分化。© 2016 Wiley Periodicals，Inc. J Biomed Mater Res Part A：104A：1687-1693，2016.
Type II collagen is reported to have the capability of guiding adipose‐derived stem cells (ADSCs) to differentiate towards a nucleus pulposus (NP)‐like phenotype. So this study aimed to establish a three‐dimensional (3D) collagen scaffold using N,N‐(3‐dimethylaminopropyl)‐N′‐ethyl carbodiimide andN‐hydroxysuccinimide (EDAC/NHS) to increase the efficiency of ADSC differentiation into NP‐like cells. Physical properties, such as porosity, biodegradation, and microstructure, and biological characteristics such as cytotoxicity, cell proliferation, and expression of relevant genes and proteins were measured to evaluate the efficacy of different scaffolds. Collagen scaffolds cross‐linked with EDAC/NHS exhibited higher biological stability, better spatial structure, and higher gene and protein expression of functional markers such as aggrecan, SOX9 and COL2 than those of other groups. Based on the results, freeze‐dried type II collagen cross‐linked with EDAC/NHS formed the best 3D scaffold, for inducing ADSC proliferation and differentiation toward a NP‐like phenotype. © 2016 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 104A: 1687–1693, 2016.