COMPLETE NUCLEOTIDE-SEQUENCE OF THE GENOMIC RNA OF SINDBIS VIRUS
COMPLETE NUCLEOTIDE-SEQUENCE OF THE GENOMIC RNA OF SINDBIS VIRUS
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DOI：
10.1016/0042-6822(84)90428-8
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发表时间：
1984-01-01
期刊：
影响因子：
3.7
通讯作者：
STRAUSS, JH
中科院分区：
文献类型：
--
作者：
STRAUSS, EG;RICE, CM;STRAUSS, JH
确定了甲病毒属的典型病毒辛德比斯病毒的基因组RNA的完整核苷酸序列。基因组长度为 11,703 个核苷酸，不包括 5'' 帽和 3'' 末端聚腺苷酸 (poly(A)) 束。在5''末端帽之后有59个核苷酸的5''非翻译核酸，其后是编码非结构多肽的7539个核苷酸的阅读框，并且除了单个蛋白石终止密码子之外它是开放的。位于分隔非结构蛋白和结构蛋白编码序列的连接区的48个非翻译碱基之后，有一个编码结构蛋白的3735个核苷酸长的开放阅读框。最后，3''非翻译区长322个核苷酸。非结构蛋白从基因组 RNA 翻译为 2 个多蛋白前体。第一个长度为 1896 个氨基酸，终止于位置 1897 的蛋白石密码子。该多蛋白经过加工产生 3 种多肽，称为 nsP1、nsP2 和 nsP3。使用可用的 N 末端氨基酸序列数据暂时定位产生这 3 种蛋白质的翻译后切割位点。在这两种情况下，切割可能发生在序列 Gly-Ala-Ala 中的 2 个 Ala 残基之间。第 4 个非结构蛋白 nsP4 是在蛋白石密码子通读产生长度为 2513 个氨基酸的第 2 个多蛋白前体时产生的，该前体也在翻译后被切割。结构蛋白是从亚基因组信息翻译而来的，该信息从核苷酸7598开始，长度为4106个核苷酸（不包括poly(A)段），并且与基因组RNA的3''末端共末端。结构蛋白也被翻译为多蛋白前体，其被裂解产生核衣壳蛋白、2种完整膜糖蛋白和2种成熟病毒颗粒中不存在的小肽。提出了基于完整核苷酸序列以及先前数据的辛德比斯病毒复制策略。
The entire nucleotide sequence of the genomic RNA of the type virus of the Alphavirus genus, Sindbis virus, was determined. The genome is 11,703 nucleotides in length, exclusive of the 5'' cap and the 3''-terminal poly(A) tract. After the 5''-terminal cap there are 59 nucleotides of 5'' nontranslated nucleic acid followed by a reading frame of 7539 nucleotides that encodes the nonstructural polypeptides and which is open except for a single opal termination codon. Following 48 untranslated bases located in the junction region which separates the nonstructural and structural protein coding sequences, there is an open reading frame 3735 nucleotides long that encodes the structural proteins. Finally, the 3'' untranslated region is 322 nucleotides long. The nonstructural proteins are translated from the genomic RNA as 2 polyprotein precursors. The first is 1896 amino acids in length and terminates at an opal codon at position 1897. This polyprotein is processed to produce 3 polypeptides called nsP1, nsP2 and nsP3. Sites of post-translational cleavage to produce these 3 proteins were tentatively located using available N-terminal amino acid sequence data. In both cases cleavage probably occurs between the 2 Ala residues in the sequence Gly-Ala-Ala. The 4th nonstructural protein, nsP4, is produced when readthrough of the opal codon produces a 2nd polyprotein precursor of length 2513 amino acids, which is also cleaved post-translationally. The structural proteins are translated from a subgenomic message which begins at nucleotide 7598, is 4106 nucleotides in length (exclusive of the poly(A) tract), and is coterminal with the 3'' end of the genomic RNA. The structural proteins are also translated as a polyprotein precursor which is cleaved to produce a nucleocapsid protein, 2 integral membrane glycoproteins, and 2 small peptides not present in the mature virion. A replication strategy for Sindbis virus based upon the complete nucleotide sequence, as well as prior data, is presented.