Flavonoids repress the production of antifungal 2,4-DAPG but potentially facilitate root colonization of the rhizobacterium Pseudomonas fluorescens
Flavonoids repress the production of antifungal 2,4-DAPG but potentially facilitate root colonization of the rhizobacterium Pseudomonas fluorescens
复制标题
类黄酮抑制抗真菌剂 2,4-DAPG 的产生，但可能促进荧光假单胞菌根部定植。
DOI：
10.1111/1462-2920.15052
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发表时间：
2020-05-15
影响因子：
5.1
通讯作者：
He，Yong-Xing
中科院分区：
文献类型：
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作者：
Yu，Xiao-Quan;Yan，Xu;He，Yong-Xing
在众所周知的豆科植物-根瘤菌共生中，豆科植物根系释放的类黄酮诱导结瘤因子的表达，并触发参与根系生长的早期植物反应。然而，植物来源的类黄酮如何影响非共生有益根际细菌的生理学仍然是未知的。在这项工作中，我们证明了类黄酮芹菜素和/或根皮素增强了蜂群运动和生产的纤维素和curli inPseudomonasfluorescens 2 P24，这两个特性是必不可少的根定殖。使用无标记定量蛋白质组学方法，我们发现芹菜素和根皮素显着降低抗真菌代谢物2，4-DAPG的生物合成，并进一步确定了一种新的类黄酮敏感TetR调节剂PhlH，其显示通过调节2，4-DAPG水解酶PhlG的表达来调节2，4-DAPG的产生。虽然具有相似的结构，芹菜素和根皮素也可以影响不同的生理特性的荧光假单胞菌2 P24，与芹菜素减少生物膜的形成和根皮素诱导蛋白质的表达参与反硝化和精氨酸发酵过程。综上所述，我们的研究结果表明，植物来源的黄酮类化合物可以被TetR调节剂PhlH在荧光假单胞菌2 P24中检测到，并作为重要的信号分子，加强植物和非共生有益根际细菌之间的互利相互作用。
In the well‐known legume–rhizobia symbiosis, flavonoids released by legume roots induce expression of the Nod factors and trigger early plant responses involved in root nodulation. However, it remains largely unknown how the plant‐derived flavonoids influence the physiology of non‐symbiotic beneficial rhizobacteria. In this work, we demonstrated that the flavonoids apigenin and/or phloretin enhanced the swarming motility and production of cellulose and curli inPseudomonas fluorescens2P24, both traits of which are essential for root colonization. Using a label‐free quantitative proteomics approach, we showed that apigenin and phloretin significantly reduced the biosynthesis of the antifungal metabolite 2,4‐DAPG and further identified a novel flavonoid‐sensing TetR regulator PhlH, which was shown to modulate 2,4‐DAPG production by regulating the expression of 2,4‐DAPG hydrolase PhlG. Although having similar structures, apigenin and phloretin could also influence different physiological characteristics ofP.fluorescens2P24, with apigenin decreasing the biofilm formation and phloretin inducing expression of proteins involved in the denitrification and arginine fermentation processes. Taken together, our results suggest that plant‐derived flavonoids could be sensed by the TetR regulator PhlH inP.fluorescens2P24 and acts as important signalling molecules that strengthen mutually beneficial interactions between plants and non‐symbiotic beneficial rhizobacteria.