Inhibition of Phytophthora parasitica and P-capsici by Silver Nanoparticles Synthesized Using Aqueous Extract of Artemisia absinthium
Inhibition of Phytophthora parasitica and P-capsici by Silver Nanoparticles Synthesized Using Aqueous Extract of Artemisia absinthium
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DOI：
10.1094/phyto-01-15-0006-r
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发表时间：
2015-09-01
期刊：
影响因子：
3.2
通讯作者：
Ali, Gul Shad
中科院分区：
文献类型：
--
作者：
Ali, Mohammad;Kim, Bosung;Ali, Gul Shad
纳米粒子在植物病害防治中的应用是一个新兴的领域，经济环保的纳米粒子合成方法得到了广泛的研究。在本项目中，我们研究了由苦艾水提取物合成的银纳米颗粒（AgNPs）对几种疫霉菌的潜力，这会导致许多经济上重要的作物病害。在微量滴定板中进行的体外剂量反应试验中，10 μ g/ml的AgNPs抑制了寄生疫霉、致病疫霉、P. pabnivora、P. cinnamomi、热带疫霉、辣椒疫霉和P. katsurae的菌丝生长。用寄生疫霉和辣椒疫霉进行的详细的体外剂量反应分析表明，用A.苦艾提取物在抑制菌丝生长、游动孢子萌发、芽管伸长和游动孢子产生方面是高度有效的（IC 50：2.1 - 8.3 μ s ml（-1））和有效的（100%）。有趣的是，AgNP处理加速了游动孢子的包囊化。与体外结果一致，在温室中进行的植物实验显示，AgNP处理防止疫霉属感染并提高植物存活率。此外，AgNP在植物实验中没有对植物生长产生任何不利影响。这些研究为AgNP防治疫霉提供了一种简单、经济、不影响植物正常生理的方法。
Application of nanoparticles for controlling plant pathogens is a rapidly emerging area in plant disease management, and nanoparticles synthesis methods that are economical and ecofriendly are extensively investigated. In this project, we investigated the potential of silver nanoparticles (AgNPs) synthesized with aqueous extract of Artemisia absinthium against several Phytophthora spp., which cause many economically important crop diseases. In in vitro dose-response tests conducted in microtiter plates, 10 mu g ml(-1) of AgNPs inhibited mycelial growth of P. parasitica, P. infestans, P. pabnivora, P. cinnamomi, P tropicalis, P. capsici, and P. katsurae. Detailed in vitro dose-response analyses conducted with P. parasitica and P. capsici revealed that AgNPs synthesized with A. absinthium extract were highly potent (IC50: 2.1 to 8.3 mu s ml(-1)) and efficacious (100%) in inhibiting mycelial growth, zoospore germination, germ tube elongation, and zoospore production. Interestingly, AgNP treatment accelerated encystment of zoospores. Consistent with in vitro results, in planta experiments conducted in a greenhouse revealed that AgNP treatments prevented Phytophthora infection and improved plant survival. Moreover, AgNP in in planta experiments did not produce any adverse effects on plant growth. These investigations provide a simple and economical method for controlling Phytophthora with AgNP without affecting normal plant physiology.