Inflammatory and metabolic responses to an intramammary lipopolysaccharide challenge in early lactating cows supplemented with conjugated linoleic acid
Inflammatory and metabolic responses to an intramammary lipopolysaccharide challenge in early lactating cows supplemented with conjugated linoleic acid
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DOI：
10.1111/jpn.12843
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发表时间：
2018-04-01
影响因子：
2.7
通讯作者：
Bruckmaier, R. M.
中科院分区：
文献类型：
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作者：
Gross, J. J.;Grossen-Roesti, L.;Bruckmaier, R. M.
补充反式-10，顺式-12共轭亚油酸（CLA）的奶牛允许营养重新分配，尽管在泌乳早期的能量缺乏，这可能是一个有益的免疫系统，太。在这项研究中，我们调查了潜在的营养节约效果的共轭亚油酸在早期泌乳奶牛低血糖浓度暴露于乳房内脂多糖（LPS）的挑战。15个多产荷斯坦奶牛暴露于乳房内LPS的挑战，在第4周的下午。8头奶牛（CLA）每天补充70克的脂质包封的CLA（6.8克反式-10，顺式-12和6.6克的顺式-9，反式-11共轭亚油酸异构体;共轭亚油酸）和7头奶牛56克的控制脂肪（CON）。每沿着直肠温度、心率和呼吸频率每30分钟采集一次血样，每小时采集一次乳汁样品，直至LPS应用后10小时。分析血浆中葡萄糖、游离脂肪酸、β-羟基丁酸酯（BHB）、皮质醇、胰岛素和胰高血糖素的浓度。在牛奶中，测定体细胞计数和乳酸脱氢酶活性。CLA的初始血糖浓度低于CON。在免疫刺激过程中，CLA的血糖浓度高于CON，CLA的BHB明显下降，而CON的BHB浓度维持在较低的水平。CLA组体温上升早，峰基温差大，下降早。总之，CLA补充早期泌乳奶牛暴露于乳房内LPS的挑战影响局部和全身免疫反应。我们假设CLA补充引发糖原储存。补充CLA的奶牛提供更多的葡萄糖，并优先使用BHB作为免疫反应期间的能量来源。更强烈的代谢和更集中的内分泌反应支持CLA补充剂的免疫调节作用。
Supplementation of dairy cows with trans-10, cis-12 conjugated linoleic acid (CLA) allows nutrient repartitioning despite an energy deficiency in early lactation, which might be a benefit for the immune system, too. In this study, we investigated potential nutrient sparing effects of CLA in early lactating cows with low plasma glucose concentrations exposed to an intramammary lipopolysaccharide (LPS) challenge. Fifteen multiparous Holstein cows were exposed to an intramammary LPS challenge in week 4 p.p. Eight cows (CLA) were supplemented daily with 70g of lipid-encapsulated CLA (6.8g trans-10, cis-12 and 6.6g of the cis-9, trans-11 CLA isomer; CLA) and seven cows with 56g of control fat (CON). Blood samples were obtained every 30min along with rectal temperature, heart and respiratory rate, and milk samples were taken hourly until 10hr after the LPS application. Plasma was analysed for concentrations of glucose, free fatty acids, beta-hydroxybutyrate (BHB), cortisol, insulin and glucagon. In milk, somatic cell count and activity of lactate dehydrogenase were determined. Initial plasma glucose concentration was lower in CLA than in CON. During the immunostimulation, CLA had higher glucose concentrations than CON, and BHB decreased distinctly in CLA, whereas CON cows maintained BHB concentration at a lower level. Body temperature in CLA increased earlier, the difference between peak and basal temperature was higher, and the decline thereafter occurred earlier. In conclusion, CLA supplementation of early lactating cows exposed to an intramammary LPS challenge affected local and systemic immune responses. We assume that CLA supplementation triggered glycogen storage. Cows supplemented with CLA provided more glucose and preferentially used BHB as an energy source during the immune response. The more intense metabolic and more concentrated endocrine responses support an immunomodulatory effect of CLA supplementation.