Relaxin promotes differentiation of human breast cancer cells MCF-7 transplanted into nude mice
Relaxin promotes differentiation of human breast cancer cells MCF-7 transplanted into nude mice
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DOI：
10.1007/s004280050435
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发表时间：
1999-11-01
期刊：
影响因子：
3.5
通讯作者：
Sacchi, TB
中科院分区：
文献类型：
--
作者：
Bani, D;Flagiello, D;Sacchi, TB
先前的研究表明，激素松弛素在体外通过调节细胞增殖和促进细胞向导管上皮表型分化而作用于人乳腺癌MCF-7细胞。本研究的目的是调查松弛素是否保留这些特性时，在体内作用于MCF-7细胞肿瘤在无胸腺裸鼠。将携带MCF-7细胞肿瘤的小鼠移植到乳腺脂肪垫下并给予雌激素以维持肿瘤生长，用松弛素（10 μ g/天）全身治疗19天。将溶剂处理的小鼠用作对照。30天后，处死小鼠，通过光镜、电镜和免疫细胞化学分析肿瘤碎片。在整个实验期间每周记录肿瘤体积的测量值。所获得的结果表明，松弛素治疗促进肿瘤细胞向肌上皮样细胞和上皮样细胞的分化，如通过细胞的超微结构特征和平滑肌肌动蛋白和钙粘蛋白的表达增加所判断的。肿瘤大小和循环细胞数量的测量表明，松弛素，在本研究中使用的剂量和暴露时间，不显着影响肿瘤生长和细胞增殖。
Previous studies showed that the hormone relaxin acts on human breast cancer MCF-7 cells in vitro by modulating cell proliferation and promoting cell differentiation toward a duct epithelial phenotype. The present study was designed to investigate whether relaxin retains these properties when acting in vivo on MCF-7 cell tumors developed in athymic nude mice. Mice bearing MCF-7 cell tumors transplanted under the mammary fat pad and estrogenized to sustain tumor growth were treated systemically with relaxin (10 mu g/day) for 19 days. Vehicle-treated mice were used as controls. Thirty days later, the mice were sacrificed and tumor fragments were analyzed by light and electron microscopy and immunocytochemistry. Measurements of tumor volume were recorded weekly for the overall experimental period. The results obtained indicate that relaxin treatment promotes differentiation of tumor cells towards both myoepithelial-like and epithelial-like cells, as judged by the ultrastructural features of the cells and by the increased expression of smooth muscle actin and cadherins. Measurements of tumor size and of the number of cycling cells show that relaxin, at the doses and times of exposure used in this study, does not significantly influence tumor growth and cell proliferation.