Increased expression of VEGF and CD31 in postradiation rectal tissue: implications for radiation proctitis.
Increased expression of VEGF and CD31 in postradiation rectal tissue: implications for radiation proctitis.
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DOI：
10.1155/2013/515048
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发表时间：
2013
影响因子：
4.6
通讯作者：
Ladas SD
中科院分区：
文献类型：
--
作者：
Karamanolis G;Delladetsima I;Kouloulias V;Papaxoinis K;Panayiotides I;Haldeopoulos D;Triantafyllou K;Kelekis N;Ladas SD
背景资料。与放射性直肠炎相关的炎症介质被部分阐明，新生血管被认为起着关键作用。目标。目的探讨血管内皮生长因子(VEGF)和CD31作为血管生成标志物在放疗后直肠组织中的表达。方法：研究方法。采用免疫组织化学方法检测11例前列腺癌患者放疗前、放疗结束时和放疗后6个月的直肠粘膜组织中血管内皮生长因子和CD31的表达。在10个高倍视野中分别计数表达血管内皮细胞和CD31的微血管。血管生成指数(VEGF-VI)和微血管密度(MVD)分别以平均每支血管数和平均每支血管数计算。并对其组织学特征进行了评价。结果。放疗结束时VEGFVI显著升高(0.17±0.15vs0.41±0.24，P=0.001)，6个月后下降。微血管密度仅在放疗后6个月显著增加(7·3±3·2比10·5±3·1，P<；0.005)。组织病理学检查显示，大部分病例在放疗结束后6个月后炎性改变消退。结论。我们的结果表明，在辐射后直肠活检标本中，新血管生成似乎与炎症有关，并构成辐射后组织损伤的重要组成部分。
Background. Inflammation mediators related to radiation proctitis are partially elucidated, and neovascularization is thought to play a key role. Objectives. To investigate the expression of vascular endothelial growth factor (VEGF) and CD31 as angiogenetic markers in postradiation rectal tissue. Methods. Rectal mucosa biopsies from 11 patients who underwent irradiation for prostate cancer were examined immunohistochemically for the expression of VEGF and CD31 at three time settings—before, at the completion of, and 6 months after radiotherapy. VEGF expressing vascular endothelial cells and CD31 expressing microvessels were counted separately in 10 high-power fields (HPFs). VEGF vascular index (VEGF-VI) and microvascular density (MVD) were calculated as the mean number of VEGF positive cells per vessel or the mean number of vessels per HPF, respectively. Histological features were also evaluated. Results. VEGF-VI was significantly higher at the completion of radiotherapy (0.17 ± 0.15 versus 0.41 ± 0.24, P = 0.001) declining 6 months after. MVD increased significantly only 6 months after radiotherapy (7.3 ± 3.2 versus 10.5 ± 3.1, P < 0.005). The histopathological examination revealed inflammatory changes at the completion of radiotherapy regressing in the majority of cases 6 months after. Conclusions. Our results showed that in postradiation rectal biopsy specimens neoangiogenesis seems to be inflammation-related and constitutes a significant postradiation component of the tissue injury.