Dynamic changes of phenolic compounds during artificial aging of soybean seeds identified by high-performance liquid chromatography coupled with transcript analysis
Dynamic changes of phenolic compounds during artificial aging of soybean seeds identified by high-performance liquid chromatography coupled with transcript analysis
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DOI：
10.1007/s00216-019-01767-5
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发表时间：
2019-05-01
影响因子：
4.3
通讯作者：
Luo, Liping
中科院分区：
文献类型：
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作者：
Cui, Meng;Wu, Dong;Luo, Liping
酚类化合物是植物中重要的生物活性物质，但对其在大豆种子老化过程中的变化研究仍然有限。本研究对大豆种子进行人工老化，利用高效液相色谱法检测酚类化合物浓度的动态变化，分析酚类代谢关键酶的基因表达。建立了大豆种子人工老化过程中19种酚类化合物的详细检测方法，这些酚类化合物的浓度都发生了显著变化。原儿茶酸、芦丁和桑缕素含量降低，大豆苷元、糖苷元、染料木素和黄芩苷含量升高。咖啡酸、表儿茶素、阿魏酸、大豆苷、染料木素和白藜芦醇的浓度先上升后下降，阿魏酸的含量在人工陈化2天后最高，其他5种酚类化合物的含量在人工陈化4天后最高。19种酚类化合物总含量在2 d时达到峰值，为2357.43 g g（-1）干重。随着人工老化时间的延长，PAL1、PAL2、PAL3、CHS7、CHS8、IFS1、IFS2、CHR1、4CL2、C4H和CHI2的相对表达量大多下调。本研究为大豆种子资源的储存和利用提供了新的见解。
Phenolic compounds are important bioactive substances in plants, but study of their alteration during soybean seed aging is still limited. In this study, we conducted artificial aging on soybean seeds, detected the dynamic changes of phenolic compound concentrations using high-performance liquid chromatography, and analyzed the gene expression of key enzymes of phenolic metabolism. A detailed method for detection of 19 phenolic compounds during artificial aging of soybean seeds was constructed, and all of these phenols significantly changed in concentration. The content of protocatechuic acid, rutin, and morin decreased, whereas that of daidzein, glycitein, genistein, and baicalin increased. The concentration of caffeic acid, epicatechin, ferulic acid, daidzin, genistin, and resveratrol first rose and then declined, and the content of ferulic acid was highest after 2 days of artificial aging, with the other five phenolic compounds showing the highest content after 4 days of artificial aging. The total content of the 19 phenolic compounds reached a peak of 2357.43 g g(-1) dry weight at 2 days. Relative expression of PAL1, PAL2, PAL3, CHS7, CHS8, IFS1, IFS2, CHR1, 4CL2, C4H, and CHI2 was mostly downregulated as the duration of artificial aging increased. This study provides novel insights into the storage and use of soybean seed resources.