Purification of a novel heterodimer from shark (Carcharhinus plumbeus) serum by gel-immobilized metal chromatography.
Purification of a novel heterodimer from shark (Carcharhinus plumbeus) serum by gel-immobilized metal chromatography.
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通过凝胶固定金属色谱法从鲨鱼（Carcharhinus plumbeus）血清中纯化新型异二聚体。
DOI：
10.1016/0305-0491(92)90371-w
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发表时间：
1992
期刊：
影响因子：
--
通讯作者：
Marchalonis,JJ
中科院分区：
文献类型：
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作者：
Vazquez-Moreno,L;Porath,J;Schluter,SF;Marchalonis,JJ
1.用凝胶固定金属亲和层析法分离沙鲨血清的50%饱和硫酸铵组分，分离得到一种新的二硫键杂二聚体，其分子质量为70 kDa。2.还原后的分子可分解为表观质量为36 kDa和24 kDa的两条链。3.这些分子是糖蛋白，通过观察到的酶糖基化后相对分子质量的降低来确定。4.通过氨基酸组成分析和N-端氨基酸比较(10个氨基酸中有7个)，表明这两条链是相互关联的。5.该分子与高等脊椎动物特有的异源二聚体的确切关系尚不清楚。6.合成了交联型琼脂糖醋酸酯，证明它是一种高效的富集剂，也是一种疏水相互作用的吸附剂。
1. Resolution of the fraction of sandbar shark (Carcharhinus plumbeus) serum that was soluble in 50% saturated ammonium sulfate by gel-immobilized metal-affinity chromatography allowed the isolation of a novel disulfide-bonded heterodimer of intact mass 70 kDa. 2. Following reduction, the molecule could be resolved into two chains of apparent mass 36 and 24 kDa. 3. The molecules were glycoproteins as determined by an observed reduction in molecular weight following enzymatic glycosylation. 4. The two separate chains were related to one another on the basis of amino-acid composition analysis and by comparison of the N-terminal amino acids (seven out of 10 identities). 5. The exact relationship of this molecule to characterized heterodimers of higher vertebrates is unknown. 6. Cross-linked agarose-acetate was synthesized and proved to be an efficient concentrating agent and also a hydrophobic interaction adsorbant.