E5 open reading frame of bovine papillomavirus type 1 encodes a transforming gene.
E5 open reading frame of bovine papillomavirus type 1 encodes a transforming gene.
复制标题
1型牛乳头瘤病毒的E5开放阅读框编码转化基因。
DOI：
10.1128/jvi.57.1.1-6.1986
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发表时间：
1986
影响因子：
5.4
通讯作者：
Lowy,DR
中科院分区：
文献类型：
--
作者：
Schiller,JT;Vass,WC;Vousden,KH;Lowy,DR
我们先前已经证明，牛乳头瘤病毒1型基因组的早期区域包含两个不重叠的片段，它们可以独立地诱导培养细胞的形态转化。E6开放阅读框编码早期区域5‘端的转化基因。第二个转化片段先前定位于从早期区域3‘端开始的2.3kb的片段。为了确定位于2.3T内的四个开放阅读框(E2、E3、E4和E5)中的哪一个编码转化基因，我们现在将一系列插入和缺失突变引入到2.3T被Harvey病毒长末端重复激活的克隆(PHLB1)中，并测试了突变体诱导焦点转化的能力。我们的结果表明，在PHLB1诱导的NIH 3T3细胞转化过程中，E5开放阅读框是必需的，而E2、E3和E4开放阅读框不是必需的。
We have previously shown that the early region of the bovine papillomavirus type 1 genome contains two nonoverlapping segments that can independently induce the morphological transformation of cultured cells. The transforming gene from the 5' end of the early region is encoded by the E6 open reading frame. The second transforming segment was previously localized to a 2.3-kilobase fragment (2.3T) from the 3' end of the early region. To determine which of the four open reading frames (E2, E3, E4, and E5) located within 2.3T encodes a transforming gene, we have now introduced a series of insertion and deletion mutations into a clone (pHLB1) in which 2.3T is activated by the Harvey viral long terminal repeat, and we tested the mutants for their ability to induce focal transformation. Our results indicate that the E5 open reading frame, which could encode a low-molecular-weight hydrophobic peptide, is required for pHLB1-induced transformation of NIH 3T3 cells, but that the E2, E3, and E4 open reading frames are not.