A platelet factor stimulating the proliferation of vascular endothelial cells. Partial purification and characterization.
A platelet factor stimulating the proliferation of vascular endothelial cells. Partial purification and characterization.
复制标题
刺激血管内皮细胞增殖的血小板因子。
DOI：
10.1016/s0014-4827(85)80022-7
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发表时间：
1985
影响因子：
3.7
通讯作者：
F. Takaku
中科院分区：
文献类型：
--
作者：
K. Miyazono;T. Okabe;S. Ishibashi;A. Urabe;F. Takaku
研究表明，血小板中含有一种新的生长因子，可在体外刺激血管内皮细胞的增殖。该因子有效地刺激了无血清内皮细胞的DNA合成和增殖率。凝胶排斥层析显示内皮细胞上至少有两个活性峰，主要峰在表观分子量20000处。等电聚焦分析表明，该因子的等电点为4.0~4.8。它被吸附在DEAE离子交换色谱柱上，并以盐梯度洗脱。该因子对热不稳定，对胰酶敏感。该活性未被包括二硫苏糖醇在内的还原剂破坏。该因子可刺激血管内皮细胞增殖，但对正常大鼠肾成纤维细胞无作用。
Platelets have been shown to contain a novel growth factor that stimulates the proliferation of vascular endothelial cells in vitro. The factor potently stimulated both DNA synthesis and proliferation rate in serum-deprived endothelial cells. Gel exclusion chromatography showed at least two peaks of activity on endothelial cells, the major peak being at an apparent molecular weight of 20000. Isoelectric focusing revealed that the pI of the factor was 4.0–4.8. It was adsorbed to a column of DEAE ion exchange chromatography and eluted with a salt gradient. The factor was heat-labile and trypsin-sensitive. The activity was not destroyed by a reducing agent including dithiothreitol. This factor stimulated the proliferation of vascular endothelial cells but was found to be inactive against normal rat kidney fibroblasts.