MEMORY B-CELLS IN T-CELL-DEPENDENT ANTIBODY-RESPONSES COLONIZE THE SPLENIC MARGINAL ZONES
MEMORY B-CELLS IN T-CELL-DEPENDENT ANTIBODY-RESPONSES COLONIZE THE SPLENIC MARGINAL ZONES
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DOI：
10.1002/eji.1830180306
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发表时间：
1988-03-01
影响因子：
5.4
通讯作者：
MACLENNAN, ICM
中科院分区：
文献类型：
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作者：
LIU, YJ;OLDFIELD, S;MACLENNAN, ICM
在用半抗原 - 蛋白质偶联物免疫后，在脾脏边缘区鉴定出了特异性结合半抗原的B细胞。边缘区B细胞对半抗原的结合似乎不是由于抗半抗原抗体的被动吸收。对于用两种半抗原（分别与血蓝蛋白偶联的2,4 - 二硝基苯（DNP）和2 - 苯噁唑酮（Ox））进行的双重免疫，结果在边缘区出现了离散的DNP结合细胞和Ox结合细胞。很少发现能同时结合这两种半抗原的细胞。边缘区的半抗原结合细胞具有其他边缘区B细胞的表型特征。它们表达表面IgM但不表达IgD。偶尔有些细胞也有表面IgG2c。所有半抗原结合细胞都具有单克隆抗体HIS 14所识别的抗原，但缺乏HIS 24和HIS 22所识别的抗原。已表明半抗原结合B细胞在进入边缘区前不久处于细胞周期中，但到达该部位后就不再处于细胞周期。一旦进入边缘区，半抗原结合细胞会在该部位停留长达2周以上。在用DNP - 血蓝蛋白再次免疫后，边缘区的DNP结合细胞（而非Ox结合细胞）消失了。同时，DNP结合细胞到达了动脉周围淋巴细胞鞘，在较小程度上也到达了淋巴滤泡。这些细胞处于活跃的细胞周期，似乎既能产生浆细胞，也能产生边缘区半抗原结合细胞。结论是，在边缘区发现的半抗原结合细胞是记忆B细胞，即它们来自经历过抗原驱动增殖的B细胞，它们不再处于细胞周期，但可通过后续暴露于抗原而被诱导重新进入细胞周期。在用半抗原 - 多糖免疫后获得了良好的抗体反应；然而，针对这些T细胞非依赖性2型抗原，边缘区没有出现半抗原结合细胞。
Specific hapten-binding B cells were identified in the splenic marginal zones following immunization with hapten-protein conjugates. Hapten binding by marginal zone B cells does not appear to be due to passive absorption of anti-hapten antibody. For double immunization with two haptens, 2,4-dinitrophenyl (DNP) and 2-phenyloxazalone (Ox) each conjugated to hemocyanin, resulting in the appearance of discrete DNP-binding cells and Ox-binding cells in the marginal zone. Very few cells were identified which bound both haptens. The hapten-binding cells in the marginal zones have a phenotype charcteristic of other marginal zone B cells. They express surface IgM but not IgD. Occasional cells also have surface IgG2c. All hapten-binding cells possessed the antigen recognized by the monoclonal antibody HIS 14 but lacked those idenitifed by HIS 24 and HIS 22. Hapten-binding B cells were shown to have been in cell cycle shortly before entering the marginal zone but were no longer in cell cycle after arriving at that site. Once in the marginal zone hapten-binding cells were shown to remain in that site for upwards of 2 weeks. Following reimmunization with DNP-hemocyanin, DNP-binding but not Ox-binding cellls were lost from the marginal zone. At the same time DNP-binding cells arrived in the periarteriolar lymphocytic sheath and to a lesser extent the follicles. These cells were in active cycle and appeared to give rise both to plasma cells and marginal zone hapten-binding cells. It is concluded that hapten-binding cells found in the amarginal zones are memory B cells i.e, they have been derived from B cells which have undergone antigen-driven proliferation, they are no longer in cell cycle but can be induced to re-enter cell cycle by subsequent exposure to antigen. Good antibody responses were obtained following immunization with hapten-polysaccharides; however, no hapten-binding cells appeared in the marginal zones in response to these T cell-independent type 2 antigens.