Hyperbaric Oxygen Therapy After Acute Thoracic Spinal Cord Injury Improvement of Locomotor Recovery in Rats
Hyperbaric Oxygen Therapy After Acute Thoracic Spinal Cord Injury Improvement of Locomotor Recovery in Rats
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DOI：
10.1097/brs.0000000000002387
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发表时间：
2018-04-15
期刊：
影响因子：
3
通讯作者：
Radaelli，Lucas
中科院分区：
文献类型：
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作者：
Falavigna，Asdrubal;Figueiro，Manuela Peletti;Radaelli，Lucas
研究设计：一项对照实验室研究。目的：本研究的目的是用轻度和中度脊髓损伤（SCI）模型分析高压氧治疗（HT）的有效性。背景资料概要：SCI可导致永久性损伤，并产生社会经济后果。运动缺陷通过两种机制发生：神经元细胞的破坏和局部炎症反应，导致缺氧。HT通过增加受伤区域的氧气而起作用。方法：对72只雌性Wistar大鼠进行胸椎椎板切除术。MASCIS撞击器在12.5 mm（n= 35）和25 mm（n= 35）高度分别用于轻度和中度SCI。在SCI后第1、7、14、21和28天通过Basso、Beattie和Bresnahan量表（BBB）评估肌肉力量。将动物随机分为5个亚组，每组7只动物：（1）对照组有SCI，无HT;（2）SCI后30分钟HT;（3）SCI后30分钟HT，每天一次，持续7天;（4）SCI后12小时HT;（5）SCI后12小时HT，每天一次，持续7天。HT在2.5大气压下进行1 hour.Results.There是一个线性关系损伤的严重程度和运动功能障碍，直到第21天，与第28天的BBB评分相似。在轻度SCI中观察到一种均匀的病变模式，与中度SCI相比，BBB的变化较低。与对照组相比，所有接受HT的动物在运动功能和组织学方面均有显著改善。无论损伤模型，动物提交到7天协议有早期改善运动功能和较小面积的组织学injure.Conclusion.本研究报告，越早HT开始后，轻度和中度SCI和更大的会话数量，更大和更早的是运动恢复和更小的是组织损伤.证据等级：N/A错误在2018年4月15日出版的Spine中，Falavigna等人的文章“急性胸脊髓损伤后的高压氧治疗：改善大鼠的运动恢复”中，作者的名字被错误拼写为Scheila Cardoso dos桑托斯，MD。正确的拼写是Scheila dos桑托斯卡多佐，医学博士。
Study Design.A controlled laboratory study.Objective.The aim of this study was to analyze the effectiveness of hyperbaric therapy (HT) using mild and moderate models of spinal cord injury (SCI).Summary of Background Data.SCI can cause permanent impairment with socioeconomic consequences. The motor deficit occurs by two mechanisms: destruction of neuronal cells and local inflammatory response, resulting in hypoxia. HT acts by increasing oxygen in the injured area.Methods.Thoracic laminectomy was performed in 72 female Wistar rats. The MASCIS impactor was used at 12.5 mm (n= 35) and 25 mm (n= 35) of height to perform, respectively, mild and moderate SCI. Muscle strength was assessed through the Basso, Beattie, and Bresnahan scale (BBB) on days 1, 7, 14, 21, and 28 after SCI. The animals were randomized into five subgroups with seven animals each:(1) control group had SCI without HT;(2) HT 30 minutes after SCI;(3) HT 30 minutes after SCI and daily for 7 days;(4) HT 12 hours after SCI; and (5) HT 12 hours after SCI and daily for 7 days. HT was performed at 2.5 atm for 1 hour.Results.There was a linear relationship between injury severity and motor deficit until day 21, with similar BBB scores on day 28. A pattern of uniform lesions was observed in the mild SCI, with lower variation of BBB when compared with moderate SCI. All animals that underwent HT had significant improvement in motor function and histology when compared with control group. Regardless of the injury model, animals submitted to 7-day protocols had an early improvement in motor function and a smaller area of histological injury.Conclusion.The present study reported that the sooner HT is begun after mild and moderate SCI and the larger the number of sessions, the greater and earlier is the motor recovery and smaller is the tissue injury.Level of Evidence: N/AErratumIn the April 15, 2018 issue of Spine in the article by Falavigna et al,“Hyperbaric Oxygen Therapy After Acute Thoracic Spinal Cord Injury: Improvement of Locomotor Recovery in Rats”, an author's name was misspelled as Scheila Cardoso dos Santos, MD. The correct spelling is Scheila dos Santos Cardoso, MD.