(-)-Epigallocatechin gallate inhibition of osteoclastic differentiation via NF-κB
(-)-Epigallocatechin gallate inhibition of osteoclastic differentiation via NF-κB
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DOI：
10.1016/j.bbrc.2009.01.007
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发表时间：
2009-02-20
影响因子：
3.1
通讯作者：
Wang, Gwo-Jaw
中科院分区：
文献类型：
--
作者：
Lin, Ru-Wei;Chen, Chung-Hwan;Wang, Gwo-Jaw
研究发现，经常喝茶的人比不喝茶的人具有更高的骨矿物质密度（BMD），并且髋部骨折的风险更低。据报道，绿茶儿茶素（例如（）-表没食子儿茶素没食子酸酯（EGCG））可增强间充质干细胞的成骨功能。然而，其对破骨细胞生成的影响仍不清楚。在这项研究中，我们研究了 EGCG 对小鼠前破骨细胞系 RAW 264.7 中 RANKL 激活破骨细胞生成和 NF-κ B 的影响。 EGCG (10-100 mu M) 显着抑制 RANKL 诱导的破骨细胞分化以及小鼠 RAW 264.7 细胞和骨髓巨噬细胞 (BMM) 中凹坑的形成。 EGCG 似乎在早期阶段就以破骨细胞分化为目标，但对破骨细胞前体没有细胞毒性作用。此外，它还显着抑制 RANKL 诱导的 NF-kappa B 转录活性和核转位。我们得出结论，EGCG 通过激活 NF-kappa B 来抑制破骨细胞生成。(C) 2009 Elsevier Inc. 保留所有权利。
People who regularly drink tea have been found to have a higher bone mineral density (BMD) and to be at less risk of hip fractures than those who do not drink it. Green tea catechins Such as ( )-epigallocatechin gallate (EGCG) have been reported to increase osteogenic functioning in mesenchymal stem cells. However, its effect on osteoclastogenesis remains unclear. In this study, we investigated the effect of EGCG on RANKL-activation osteoclastogenesis and NF-kappa B in RAW 264.7, a murine preosteoclast cell line. EGCG (10-100 mu M) significantly suppressed the RANKL-induced differentiation of osteoclasts and the formation of pits in murine RAW 264.7 cells and bone marrow macrophages (BMMs). EGCG appeared to target osteoclastic differentiation at an early stage but had no cytotoxic effect on osteoclast precursors. In addition, it significantly inhibited RANKL-induced NF-kappa B transcriptional activity and nuclear translocation. We conclude that EGCG inhibits osteoclastogenesis through its activation of NF-kappa B. (C) 2009 Elsevier Inc. All rights reserved.