Sperm factors related to in vitro and in vivo porcine fertility
Sperm factors related to in vitro and in vivo porcine fertility
复制标题
DOI：
10.1016/j.theriogenology.2004.09.023
复制
发表时间：
2005-01-15
期刊：
影响因子：
2.8
通讯作者：
Gadea, J
中科院分区：
文献类型：
--
作者：
Gadea, J
当采用人工授精时，精子受精能力的预测对于种猪群具有重要的经济意义。精液评估的经典方法通常测量精子浓度、前进活力、活细胞百分比和顶体形态。这些检测方法在预测精子生育力方面效果不佳，因为只能检测到质量明显较差的样本。测量某些精子功能的新精子测试的开发是解决这个问题的尝试。另一方面，透明带的结合和穿透是精子在受精过程中必须克服的最重要的障碍之一。此外，与卵母细胞质膜的相互作用似乎可以解释可育公猪精子受精潜力的大部分变异性。因此，研究精子因素与体外生育力之间的关系可能是一个很好的策略，并且包括配子相互作用研究在内的分析可能会比常规的精液实验室评估提供更好的方法来预测男性生育力。这篇综述将讨论稀释精液和冻融精液的体外和体内精子因素与生育力（AI试验）之间的关系。我们还将尝试分析与公猪精子测试解释相关的问题和局限性。 (C) 2004 Elsevier Inc. 保留所有权利。
The prediction of sperm fertilizing ability has great economic importance for breeding herds when artificial insemination is used. Classical methods of semen evaluation generally measure the sperm concentration, progressive motility, percentage of viable cells, and acrosome morphology. These assays are poor in predicting sperm fertility, because only the samples with markedly poor quality can be detected. The development of new sperm tests that measure certain sperm functions is an attempt to solve this problem. On the other hand, the binding and penetration of the zona pellucida is one of the most important barriers the spermatozoa must overcome in the fertilization process. Also, the interaction with the oocyte plasma membrane appears to explain much of the variability in sperm fertilizing potential among fertile boars. Thus, the study of the relationship between sperm factors and in vitro fertility may be a good strategy and assays that include a study of gamete interaction may lead to a better way to predict male fertility than the routine laboratory evaluation of semen.This review will discuss the relationships between sperm factors and fertility in vitro and in vivo (AI trial) with both diluted and frozen-thawed semen. We will also try to analyze the problems and limitations related to the interpretation of boar sperm tests. (C) 2004 Elsevier Inc. All rights reserved.