HUMAN FOAMY VIRUS-INFECTION ACTIVATES CLASS-I MAJOR HISTOCOMPATIBILITY COMPLEX ANTIGEN EXPRESSION
HUMAN FOAMY VIRUS-INFECTION ACTIVATES CLASS-I MAJOR HISTOCOMPATIBILITY COMPLEX ANTIGEN EXPRESSION
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DOI：
10.1099/0022-1317-76-3-661
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发表时间：
1995-03-01
影响因子：
3.8
通讯作者：
EMANOILRAVIER, R
中科院分区：
文献类型：
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作者：
COLAS, S;BOURGE, JF;EMANOILRAVIER, R
我们研究了人泡沫病毒（HEV）感染对人主要组织相容性复合体分子表达的影响。我们的数据表明，在体外HFV感染的U373-MG胶质母细胞瘤细胞的结果增加表达的I类人类白细胞抗原（HLA）和转录。瞬时转染试验的质粒含有报告基因氯霉素乙酰转移酶驱动的不同5'缺失的HLA-A11类I启动子允许识别的顺式作用元件参与这一调节。HFV感染对HLA I类启动子有两种相反的作用：通过位于外显子1上游-525至-335区域的正调控元件反式激活HLA-A11启动子，以及下调由-335至-205 I类启动子区域驱动的转录活性。另外的实验数据表明，HFV对HLA I类表达的影响不是由干扰素途径介导的。
We examined the effect of human foamy virus (HEV) infection on the expression of human major histocompatibility complex molecules. Our data show that in vitro HFV infection of U373-MG glioblastoma cells results in increased expression of class I human leukocyte antigen (HLA) and transcripts. Transient transfection assays of plasmids containing the reporter gene chloramphenicol acetyl transferase driven by different 5' deletions of the HLA-A11 class I promoter allowed identification of cis-acting elements involved in this regulation. HFV infection has two opposite effects on the HLA class I promoter: transactivation of the HLA-A11 promoter through a positive regulatory element located in the -525 to -335 region upstream of exon 1 and down-regulation of transcriptional activity driven by the -335 to -205 class I promoter region. Additional experimental data indicate that the effect of HFV on HLA class I expression is not mediated by the interferon pathway.