Metabolism of constituents in Huangqin-Tang, a prescription in traditional Chinese medicine, by human intestinal flora
Metabolism of constituents in Huangqin-Tang, a prescription in traditional Chinese medicine, by human intestinal flora
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DOI：
10.1248/bpb.25.558
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发表时间：
2002-05-01
影响因子：
2
通讯作者：
Ye, WH
中科院分区：
文献类型：
--
作者：
Zuo, F;Zhou, ZM;Ye, WH
在研究黄芩汤有效成分经人肠道植物群代谢的过程中，将黄芩汤及各单味中药分别与人粪便悬液孵育。采用本实验室建立的高效液相色谱法（HPLC），对复方和各单味药的代谢产物进行了定性和定量分析。结果表明黄芩汤中黄芩苷等成分（黄芩素7-葡糖苷酸; BG），汉黄芩苷（Wogoninoglucuronide; WG）、甘草中的木脂素-A-葡萄糖醛酸苷（oroxylin-A-glucuronide，OG）、甘草中的芍药苷（paconiflorin，PF）、甘草苷（liquiritin（甘草素4 '-O-葡萄糖苷; LG），异甘草素异甘草素4-葡萄糖苷;从甘草中提取的甘草素（ILG）和甘草酸（GL）分别转化为它们的代谢产物黄芩素（B）、汉黄芩素（W）、芍药甙-A（O）、芍药甙-I（PM-I）、甘草素（L）、异甘草素（IL）和甘草次酸（GA）的含量黄芩汤和各单味药煎液与肠道植物群孵育后，代谢产物的含量显著增加。但与单味药相比，复方中BG、WC、OG、LG和ILG的转化均得到促进。PF和GL的活性受到抑制。以上结果表明，HIF可将多种中药的苷类化合物转化为苷元，并改善了复方中大部分苷类化合物的代谢。
In the course of studies on the metabolism of active components of Huangqin-Tang (sic) by human intestinal flora (HIF), Huangqin-Tang and all individual herbs in the decoctions were incubated with a human fecal suspension separately. By using a high-performance liquid chromatographic (HPLC) method which was previously established in our laboratory, the metabolites in both the compound prescription and all the single herb decoctions were identified and determined both qualitatively and quantitatively. We found that the constituents of Huangqin-Tang, incluing baicalin (baicalein 7-glucuronide; BG), wogonoside (wogoninoglucuronide; WG), oroxylin-A-glucuronide (OG) from Scutellariae Radix, paconiflorin (PF) from Paeoniae Radix, liquiritin (liquiritigenin 4'-O-glucoside; LG), isoliquirtin (isoliquiritigenin 4-glucoside; ILG) and glycyrrhizic acid (GL) from Glycyhhizea Radix, were converted to their metabolites baicalein (B), wogonin (W), oroxylin-A (O), paeonimetabolin-I (PM-I), liquiritigenin (L), isoliquiritigenin (IL) and glycyrrhetinic acid (GA) by THE The contents of the metabolites in Huangqin-Tang and in each single herb decoction increased significantly after incubation with intestinal flora. Comparing with single herb decoctions, the transformation of BG, WC, OG, LG and ILG in the compound prescription was promoted, however. that of PF and GL was inhibited. All the results suggested that the glycosides of many medicinal herbs could be converted to aglycones by HIF, and the metabolism of most glycosides was improved in the compound prescription.