Constitutive expression and delayed light response of casein kinase Iε and Iδ mRNAs in the mouse suprachiasmatic nucleus
Constitutive expression and delayed light response of casein kinase Iε and Iδ mRNAs in the mouse suprachiasmatic nucleus
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DOI：
10.1002/jnr.1114
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发表时间：
2001-06-15
影响因子：
4.2
通讯作者：
Okamura, H
中科院分区：
文献类型：
--
作者：
Ishida, Y;Yagita, K;Okamura, H
酪蛋白激酶Iε（CKIε）和酪蛋白激酶Iδ（CKIδ）使生物钟振荡的mPER蛋白磷酸化，并且在产生昼夜节律的转录（后）翻译反馈回路中起关键作用。在本研究中，检测了小鼠生物钟中枢视交叉上核（SCN）中CKIε和CKIδ mRNA的表达谱。在光照、黑暗和持续黑暗条件下，视交叉上核中均持续表达中等水平的CKIε和CKIδ mRNA。这一发现支持了如下假说：CKI可能像果蝇的同源物doubletime一样，对mPER蛋白进入细胞核形成一个恒定的阈值。此外，我们证明了在主观夜间的光照会诱导视交叉上核中CKIε和CKIδ mRNA延迟增加，CKIε和CKIδ蛋白可能在光诱导的相移中起作用。（C）2001威利 - 利斯公司
Casein kinase I epsilon (CKI epsilon) and casein kinase I delta (CKI delta) phosphorylate clock oscillating mPER proteins, and play a key role in the transcription (post)translation feedback loop that generates circadian rhythm. In the present study, the expression profiles of CKI epsilon of and CKI delta mRNAs were examined in the mice clock center, suprachiasmatic nucleus (SCN), Moderate levels of CKI epsilon and CKI delta mRNAs were constantly expressed in the SCN in both light, dark and constant dark conditions. This finding supports the hypothesis that CKI may form a constant threshold to the nuclear entry of mPER proteins as in the Drosophila homologue, double-time. Further, we demonstrated that the light exposure at subjective night induced a delayed increase in CKI epsilon and CKI delta mRNAs in the SCN, CKI epsilon and CKI delta proteins may play a role on light-induced phase-shift. (C) 2001 Wiley-Liss, Inc.