Mechanism of shape determination in motile cells
Mechanism of shape determination in motile cells
复制标题
DOI：
10.1038/nature06952
复制
发表时间：
2008-05-22
期刊：
影响因子：
64.8
通讯作者：
Theriot, Julie A.
中科院分区：
文献类型：
--
作者：
Keren, Kinneret;Pincus, Zachary;Theriot, Julie A.
运动细胞的形状是由许多动态过程决定的，这些动态过程在空间和时间上跨越几个数量级，从亚秒级时间尺度的肌动蛋白单体的局部聚合到可能持续数小时的全局细胞尺度几何形状。由于涉及众多组件及其相互作用的复杂性，理解细胞中形状决定的机制已被证明极具挑战性。在这里，我们利用自然的表型变异在一个大的人口的能动上皮角膜细胞从鱼（Hypsophrys nicaraguensis），揭示机制的形状决定。我们发现，细胞栖息在一个低维的，高度相关的光谱可能的功能状态。我们进一步表明，在一个不可扩展的膜袋肌动蛋白网络的铣削模型可以定量概括这一频谱和预测细胞的形状和速度。我们的模型提供了一个简单的生物化学和生物物理学的基础上观察到的运动细胞的形态和行为。
The shape of motile cells is determined by many dynamic processes spanning several orders of magnitude in space and time, from local polymerization of actin monomers at subsecond timescales to global, cell-scale geometry that may persist for hours. Understanding the mechanism of shape determination in cells has proved to be extremely challenging due to the numerous components involved and the complexity of their interactions. Here we harness the natural phenotypic variability in a large population of motile epithelial keratocytes from fish (Hypsophrys nicaraguensis) to reveal mechanisms of shape determination. We find that the cells inhabit a low-dimensional, highly correlated spectrum of possible functional states. We further show that a model of actin network treadmilling in an inextensible membrane bag can quantitatively recapitulate this spectrum and predict both cell shape and speed. Our model provides a simple biochemical and biophysical basis for the observed morphology and behaviour of motile cells.