[Effect of inhibitors of phospholipase A(2); on the metastasis potentials of human ovarian cancer cells].
[Effect of inhibitors of phospholipase A(2); on the metastasis potentials of human ovarian cancer cells].
复制标题
[磷脂酶A(2)抑制剂的作用；
DOI：
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发表时间：
2010-10
期刊：
影响因子：
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通讯作者：
李宇
中科院分区：
文献类型：
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作者：
周熙惠;张龙;杨亚;李宇
目的
寻找卵巢癌（OC）转移的抑制剂非常重要。 OC 细胞 (OCC) 产生溶血磷脂酸 (LPA)，促进 OC 的发展。磷脂酶A(2)； (PLA(2);) 负责 LPA 生产。本研究旨在探讨PLA(2)的效果； OC 转移抑制剂。
方法
通过Transwell实验测量细胞迁移和粘附。 PLA(2)的表达和激活；通过Western blot检测。通过 ECIS 检测到腹膜单层的侵袭。 LPA浓度通过质谱测量。通过体内OC模型检测转移。
结果
(1) LPA在体外以剂量依赖性方式促进SKOV3的迁移和粘附； (2) iPLA(2)；和 cPLA(2)；在 SKOV3 中高表达并激活。 (3)AACOCF3和HELSS显着抑制SKOV3分泌LPA； (4)AACOCF3和HELSS显着抑制mude小鼠模型中SKOV3的远处转移。
结论
LPA显着增强SKOV3的迁移、粘附和侵袭。 AACOCF3和HELSS显着抑制SKOV3的LPA分泌、迁移和侵袭。它们还在体内抑制了 SKOV3 的远处转移。我们的数据表明 AACOCF3 和 HELSS 可能是 OC 转移的潜在抑制剂。
AIM
it is important to find inhibitors for metastasis of ovarian cancer (OC). OC cells (OCC) produce Lysophosphophatidic acid (LPA), which promotes the OC development. Phospholipase A(2); (PLA(2);) is responsible for LPA production. This study is to investigate the effect of PLA(2); inhibitors on the metastasis of OC.
METHODS
cell migration and adhesion were measured by Transwell assay. The expression and activation of PLA(2); were detected by Western blot. Invasion into the peritoneal monolayer was detected through ECIS. LPA concentration was measured by mass spectrum. Metastasis was detected through in vivo OC model.
RESULTS
(1) LPA promoted the migration and adhesion of SKOV3 in a dose-dependent manner in vitro; (2) iPLA(2); and cPLA(2); expressed highly and activated in SKOV3. (3)AACOCF3 and HELSS significantly inhibited LPA secretion from SKOV3; (4)AACOCF3 and HELSS significantly inhibited the distant metastasis of SKOV3 in mude mice model.
CONCLUSION
LPA significantly enhanced the migration, adhesion and invasion of SKOV3. AACOCF3 and HELSS significantly inhibited the LPA secretion, migration and invasion of SKOV3. They also inhibited the distant metastasis of SKOV3 in vivo. Our data suggest AACOCF3 and HELSS may be the potential inhibitors of metastasis of OC.