Effect of CO2 Concentration on Carbonic Anhydrase and Ribulose-1,5-Bisphosphate Carboxylase/Oxygenase Expression in Pea
Effect of CO2 Concentration on Carbonic Anhydrase and Ribulose-1,5-Bisphosphate Carboxylase/Oxygenase Expression in Pea
复制标题
CO2 浓度对豌豆碳酸酐酶和 1,5-二磷酸核酮糖羧化酶/加氧酶表达的影响
DOI：
10.1104/pp.112.2.569
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发表时间：
1996
影响因子：
2.7
通讯作者：
J. R. Coleman
中科院分区：
文献类型：
--
作者：
N. Majeau;J. R. Coleman
以豌豆（Pisum sativum cv Little Marvel）叶片为材料，研究了CO2浓度对碳酸酐酶（CA）和核酮糖-1，5-二磷酸羧化酶/加氧酶（Rubisco）表达的影响。酶的活性和它们的转录水平降低植物生长在1000 [mu]L/L CO2与植物生长在环境空气中相比。在160 [mu]L/L CO2的生长似乎也降低了rbcS（编码Rubisco小亚基的基因）和ca（编码CA的基因）的稳态转录水平;然而，rbcS转录物在此浓度下降低的程度更大。Rubisco活性略低，在160 [mu]L/L CO2生长的植物，CA活性显着高于在空气中生长的植物观察。转移植物从1000 [mu]L/L到空气中的CO2水平导致在两个CA和rbcS转录丰度在完全展开的叶片中的快速增加，其次是酶活性的增加。从空气中转移到高CO2浓度的植物似乎调节转录丰度和酶活性不太快。叶片碳水化合物水平也在植物中连续生长在高和环境CO2，并在生长条件的变化，迅速改变钙和rbcS转录丰度和酶的活性进行了研究。
The effect of external CO2 concentration on the expression of carbonic anhydrase (CA) and ribulose-1,5-bisphosphate carboxylase/oxygenase (Rubisco) was examined in pea (Pisum sativum cv Little Marvel) leaves. Enzyme activities and their transcript levels were reduced in plants grown at 1000 [mu]L/L CO2 compared with plants grown in ambient air. Growth at 160 [mu]L/L CO2 also appeared to reduce steady-state transcript levels for rbcS, the gene encoding the small subunit of Rubisco, and for ca, the gene encoding CA; however, rbcS transcripts were reduced to a greater extent at this concentration. Rubisco activity was slightly lower in plants grown at 160 [mu]L/L CO2, and CA activity was significantly higher than that observed in air-grown plants. Transfer of plants from 1000 [mu]L/L to air levels of CO2 resulted in a rapid increase in both ca and rbcS transcript abundance in fully expanded leaves, followed by an increase in enzyme activity. Plants transferred from air to high-CO2 concentrations appeared to modulate transcript abundance and enzyme activity less quickly. Foliar carbohydrate levels were also examined in plants grown continuously at high and ambient CO2, and following changes in growth conditions that rapidly altered ca and rbcS transcript abundance and enzyme activities.