Jaw1/LRMP is associated with the maintenance of Golgi ribbon structure
Jaw1/LRMP is associated with the maintenance of Golgi ribbon structure
复制标题
DOI：
10.1093/jb/mvad004
复制
发表时间：
2023-01-23
影响因子：
2.7
通讯作者：
Nishikawa，Atsushi
中科院分区：
文献类型：
--
作者：
Okumura，Wataru;Tadahira，Kazuko;Nishikawa，Atsushi
Jaw1/LRMP是一种膜蛋白，定位于内质网和外核膜。此前，我们揭示了Jaw1通过作为Klarsicht/ANC-1/Syne/Homology(Kash)蛋白与微管相互作用来维持核的形状。一些Kash蛋白的缺失导致高尔基体和细胞核的位置和形状的缺陷，但Jaw1的缺失对高尔基体的影响知之甚少。在这里，我们发现siRNA介导的Jaw1缺失导致黑色素瘤细胞内高尔基体碎裂和无序的带状结构，并伴随着高尔基体源微管网络定位的改变。因此，我们认为Jaw1是一种与微管网络相关的维持高尔基带结构的新蛋白质。
Jaw1/LRMP is a membrane protein that is localized to the endoplasmic reticulum and outer nuclear membrane. Previously, we revealed that Jaw1 functions to maintain nuclear shape by interacting with microtubules as a Klarsicht/ANC-1/Syne/homology (KASH) protein. The loss of several KASH proteins causes defects in the position and shape of the Golgi apparatus as well as the nucleus, but the effects of Jaw1 depletion on the Golgi apparatus were poorly understood. Here, we found that siRNA-mediated Jaw1 depletion causes Golgi fragmentation with disordered ribbon structure in the melanoma cell, accompanied by the change in the localization of the Golgi-derived microtubule network. Thus, we suggest that Jaw1 is a novel protein to maintain the Golgi ribbon structure, associated with the microtubule network.