Oral administration of undenatured native chicken type II collagen (UC-II) diminished deterioration of articular cartilage in a rat model of osteoarthritis (OA)
Oral administration of undenatured native chicken type II collagen (UC-II) diminished deterioration of articular cartilage in a rat model of osteoarthritis (OA)
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DOI：
10.1016/j.joca.2017.08.013
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发表时间：
2017-12-01
影响因子：
7
通讯作者：
Lane, N. E.
中科院分区：
文献类型：
--
作者：
Bagi, C. M.;Berryman, E. R.;Lane, N. E.
目的：研究未变性的天然鸡II型胶原(UC-II)对骨关节炎(OA)大鼠关节软骨过度恶化的预防作用。方法：20只雄性大鼠采用内侧半月板部分切除(PMMT)手术诱导骨关节炎模型。术后即刻给予赋形剂10只，另10只大鼠每日口服UC-II 0.66 mg/kg，共8周。另取10只幼年大鼠作为正常对照，另10只接受假手术。研究终点包括前后腿的负重能力，血清骨和软骨代谢的生物标志物，胫骨干骺端和干骺端的软骨下和松质骨的分析，以及胫骨内侧平台的软骨病理。结果：PMMT手术在胫骨内侧平台产生了中度的骨关节炎。具体地说，关节软骨的退化对手术肢体的负重能力有负面影响。即刻应用UC-II保留了损伤小腿的承重能力，保留了胫骨干骺端松质骨的完整性，限制了过度的骨赘形成和关节软骨的恶化。结论：临床相关的每日剂量的UC-II在损伤后即刻应用，可以改善损伤膝关节的机械功能，防止关节软骨的过度恶化。(三)2017年提交人(S)。由爱思唯尔有限公司代表国际骨性关节炎研究会出版。
Objective: The aim of this study was to determine the ability of undenatured native chicken type II collagen (UC-II) to prevent excessive articular cartilage deterioration in a rat model of osteoarthritis (OA).Methods: Twenty male rats were subjected to partial medial meniscectomy tear (PMMT) surgery to induce OA. Immediately after the surgery 10 rats received vehicle and another 10 rats oral daily dose of UC-II at 0.66 mg/kg for a period of 8 weeks. In addition 10 naive rats were used as an intact control and another 10 rats received sham surgery. Study endpoints included a weight-bearing capacity of front and hind legs, serum biomarkers of bone and cartilage metabolism, analyses of subchondral and cancellous bone at the tibial epiphysis and metaphysis, and cartilage pathology at the medial tibial plateau using histological methods.Results: PMMT surgery produced moderate OA at the medial tibial plateau. Specifically, the deterioration of articular cartilage negatively impacted the weight bearing capacity of the operated limb. Immediate treatment with the UC-II preserved the weight-bearing capacity of the injured leg, preserved integrity of the cancellous bone at tibial metaphysis and limited the excessive osteophyte formation and deterioration of articular cartilage.Conclusion: Study results demonstrate that a clinically relevant daily dose of UC-II when applied immediately after injury can improve the mechanical function of the injured knee and prevent excessive deterioration of articular cartilage. (C) 2017 The Author(s). Published by Elsevier Ltd on behalf of Osteoarthritis Research Society International.