The induction of macrophage spreading by factor B of the properdin system
The induction of macrophage spreading by factor B of the properdin system
复制标题
备解素系统的B因子诱导巨噬细胞扩散
DOI：
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发表时间：
1978
影响因子：
15.3
通讯作者：
Z. Cohn
中科院分区：
文献类型：
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作者：
Otto G6tze;C Bianco;Z. Cohn
未受刺激的小鼠腹腔巨噬细胞附着在玻璃基质上，对备解素系统的活化人因子B（BB）作出反应，但对天然因子B不作出反应，其表面积迅速扩散并随之增加。通过离子交换层析、分子筛层析和碱性聚丙烯酰胺凝胶电泳对Bb蛋白进行纯化，发现Bb蛋白的分布与细胞铺展活性有很好的相关性。1.6微克纯化的Bb足以在37 ℃下1-2小时内在50%的5 × 10（4）玻璃附着的巨噬细胞中诱导扩散。用二异丙基氟磷酸盐处理Bb表明，启动巨噬细胞扩散需要丝氨酸蛋白酶Bb的完整催化位点。因子B参与诱导快速细胞扩散也可以在自体系统中间接证明，其中小鼠B的抗血清的F（ab '）2片段防止小鼠巨噬细胞响应补体活化的小鼠血清而扩散。这些实验表明因子B和补体结合的替代途径在单核吞噬细胞定位于炎症区域中的作用。
Unstimulated mouse peritoneal macrophages attached to a glass substratum responded to activated human factor B (Bb) of the properdin system but not to native factor B with rapid spreading and a concomitant increase in their apparent surface area. Excellent correlation of the distribution of Bb protein and cell-spreading activity was found upon purification of Bb by ion-exchange and molecular seive chromatography and alkaline polyacrylamide gel electrophoresis. 1.6 microgram of purified Bb was sufficient to induce spreading in 50% of 5 x 10(4) glass attached macrophages within 1-2 h at 37 degrees C. Treatment of Bb with di-isopropyl-fluorophosphate indicated that the intact catalytic site of the serine-proteinase Bb was required for the initiation of macrophage spreading. The involvement of factor B in the induction of rapid cell spreading could also be indirectly demonstrated in an autologous system in which F(ab')2 fragments of an antiserum to mouse B prevented mouse macrophages from spreading in response to complement-activated mouse serum. These experiments suggest a role for factor B and the alternative pathway of complement fixation in the localization of mononuclear phagocytes to areas of inflammation.