Bacterial mixture identification using Raman and surface-enhanced Raman chemical imaging
Bacterial mixture identification using Raman and surface-enhanced Raman chemical imaging
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DOI：
10.1002/jrs.2601
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发表时间：
2010-12-01
影响因子：
2.5
通讯作者：
Tripathi, Ashish
中科院分区：
文献类型：
--
作者：
Guicheteau, Jason;Christesen, Steven;Tripathi, Ashish
正常拉曼和表面增强拉曼散射（SERS）识别和检测细菌的能力在最近的研究中取得了巨大的成功。在细菌样品中加入纳米银不仅可以增强拉曼信号，还可以抑制与生物材料相关的天然荧光。在本报告中，拉曼化学成像（RCI）用于分析单个细菌和孢子和营养细胞的复杂混合物。RCI使用拉曼相机的每个像素或一个像素组（BPG）作为一个独立的拉曼光谱仪，可以收集空间分辨的拉曼光谱。该技术的优势主要在于分析复杂背景下的样品，而不需要物理分离或纯化样品。使用化学成像拉曼显微镜，我们比较正常的RCI和sers辅助的细菌混合物的化学成像。在这两种情况下，我们都能够在拉曼显微镜的视野中区分单个细菌，图像采集时间减少了60倍，SERS化学成像的信噪比比比正常RCI增加了10倍。2010年由John Wiley & Sons出版。
The ability of normal Raman and surface-enhanced Raman scattering (SERS) to identify and detect bacteria has shown great success in recent studies. The addition of silver nanoparticles to bacterial samples not only results in an enhanced Raman signal, but it also suppresses the native fluorescence associated with biological material. In this report, Raman chemical imaging (RCI) was used to analyze individual bacteria and complex mixtures of spores and vegetative cells. RCI uses every pixel or a binned pixel group (BPG) of the Raman camera as an independent Raman spectrograph, allowing collection of spatially resolved Raman spectra. The advantage of this technique resides primarily in the analysis of samples in complex backgrounds without the need for physically isolating or purifying the sample. Using a chemical imaging Raman microscope, we compare normal RCI to SERS-assisted chemical imaging of mixtures of bacteria. In both cases, we are able to differentiate single bacterium in the Raman microscope's field of view, with a 60-fold reduction in image acquisition time and a factor of 10 increase in the signal-to-noise ratio for SERS chemical imaging over normal RCI. Published in 2010 by John Wiley & Sons, Ltd.