Widely spread butyrylcholinesterase can hydrolyze acetylcholine in the normal and Alzheimer brain
Widely spread butyrylcholinesterase can hydrolyze acetylcholine in the normal and Alzheimer brain
复制标题
DOI：
10.1006/nbdi.2001.0462
复制
发表时间：
2002-02-01
影响因子：
6.1
通讯作者：
Quinn, B
中科院分区：
文献类型：
--
作者：
Mesulam, M;Guillozet, A;Quinn, B
背景：丁酰胆碱酯酶（BChE），也称为“伪”或“非神经元”胆碱酯酶，传统上被认为具有受限的中枢神经系统分布，并且在胆碱能传递中发挥很小（如果有的话）的作用。目的：以更灵敏的方法重新分析BChE在人脑中的作用。方法：用乙酰胆碱酯酶和 BChE 组织化学检查三个大脑。用明视野显微镜和暗视野显微镜检查切片。结果：本实验中使用的组织化学参数表明，BChE 活性存在于已知接受胆碱能输入的所有海马和颞叶新皮质区域中。在所有这些位置，BChE 酶都可以水解乙酰胆碱替代物乙酰硫代胆碱。海马和新皮质 BChE 的很大一部分似乎位于神经胶质细胞及其突起内。结论：丁酰胆碱酯酶在胆碱能传递中的作用可能比之前推测的更大，这使得 BChE 抑制成为阿尔茨海默病的重要治疗目标。结果还表明，神经胶质细胞在胆碱能传递中的作用可能类似于其在谷氨酸能传递中众所周知的作用。 (C) 2002 年爱思唯尔科学（美国）。
Background: Butyrylcholinesterase (BChE), also known as the "pseudo" or "non-neuronal" cholinesterase, is traditionally thought to have a restricted CNS distribution and to play little, if any, role in cholinergic transmission. Objective: To reanalyze the role of BChE in the human brain with more sensitive methodology. Methods: Three brains were examined with acetylcholinesterase and BChE histochemistry. The sections were examined with bright- and dark-field microscopy. Results: The histochemical parameters used in the present experiments showed that BChE activity was present in all hippocampal and temporal neocortical areas known to receive cholinergic input. At all of these locations, the BChE enzyme could hydrolyze the acetylcholine surrogate acetylthiocholine. A substantial portion of the hippocampal and neocortical BChE appeared to be located within neuroglia and their processes. Conclusions: Butyrylcholinesterase may have a greater role in cholinergic transmission than previously surmised, making BChE inhibition an important therapeutic goal in Alzheimer's disease. The results also suggest that the role of neuroglia in cholinergic transmission may be analogous to their well known role in glutamatergic transmission. (C) 2002 Elsevier Science (USA).