Cloning and bioinformatic analysis of lovastatin biosynthesis regulatory gene lovE
Cloning and bioinformatic analysis of lovastatin biosynthesis regulatory gene lovE
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DOI：
10.3760/cma.j.issn.0366-6999.2009.15.016
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发表时间：
2009-08-05
影响因子：
6.1
通讯作者：
Li Hao-ming
中科院分区：
文献类型：
--
作者：
Huang Xin;Li Hao-ming
背景洛伐他汀是治疗高脂血症的有效药物。方法根据Genebank中洛伐他汀合成酶基因序列设计引物，从土曲霉基因组DNA中扩增并克隆洛伐他汀合成酶基因lovE，并对其编码蛋白进行结构与功能分析。结果从土曲霉基因组DNA中扩增出约1500 bp的目的片段lovE，并预测了LovE蛋白的二级结构和三维结构。
Background Lovastatin is an effective drug for treatment of hyperlipidemia. This study aimed to clone lovastatin biosynthesis regulatory gene lovE and analyze the structure and function of its encoding protein.Methods According to the lovastatin synthase gene sequence from genebank, primers were designed to amplify and clone the lovastatin biosynthesis regulatory gene lovE from Aspergillus terrus genomic DNA. Bioinformatic analysis of lovE and its encoding animo acid sequence was performed through internet resources and software like DNAMAN.Results Target fragment lovE, almost 1500 bp in length, was amplified from Aspergillus terrus genomic DNA and the secondary and three-dimensional structures of LovE protein were predicted.Conclusion In the lovastatin biosynthesis process lovE is a regulatory gene and LovE protein is a GAL4-like transcriptional factor.