CHARACTERIZATION OF THE TRYPSIN-LIKE PROTEASE (HA-TLP2) CONSTITUTIVELY EXPRESSED IN THE INTEGUMENT OF THE COTTON BOLLWORM, Helicoverpa armigera
CHARACTERIZATION OF THE TRYPSIN-LIKE PROTEASE (HA-TLP2) CONSTITUTIVELY EXPRESSED IN THE INTEGUMENT OF THE COTTON BOLLWORM, Helicoverpa armigera
复制标题
棉铃虫体皮中组成型表达的胰蛋白酶样蛋白酶 (HA-TLP2) 的表征
DOI：
10.1002/arch.20324
复制
发表时间：
2009-10-01
影响因子：
2.2
通讯作者：
Zhao, Xiao-Fan
中科院分区：
文献类型：
--
作者：
Liu, Yang;Sui, Yi-Peng;Zhao, Xiao-Fan
Trypsins belong to the serine endoproteases. They are the most important proteases in insects because of their key roles in food digestion and zymogens activation. But there has been little study of the trypsins in the integuments of insects. In this work, we cloned a trypsin-like protease gene from Helicoverpa armigera and named it trypsin-life protease 2 (Ha-TLP2). Semi-quantitative reverse transcription PCR analysis showed that Ha-TLP2 is constitutively expressed in the integument and can be down-regulated by 20-hydroxyecdysone (20E) and up-regulated by the juvenile hormone (JH) analog methoprene. Immunohistochemistry showed that Ha-TLP2 is located not only in the epidermis, but also in new and old cuticles. Immunoblotting and gelatin-SDS-PAGE revealed that Ha-TLP2 is constitutively expressed with activity in the integument during larval feeding, molting, and metamorphosis. is. This evidence suggests that Ha-TLP2 is involved in the remodeling of the integument. (C) 2009 Wiley Periodicals, Inc.
