Control of dental-derived induced pluripotent stem cells through modified surfaces for dental application
Control of dental-derived induced pluripotent stem cells through modified surfaces for dental application
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DOI：
10.1080/00016357.2017.1303847
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发表时间：
2017-01-01
影响因子：
2
通讯作者：
Park, Young-Bum
中科院分区：
文献类型：
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作者：
Choi, Hyunmin;Park, Kyu-Hyung;Park, Young-Bum
目的：研究牙干细胞诱导多能干细胞在不同表面处理的钛盘上的初始粘附和分化潜能。材料和方法：利用仙台病毒介导4重编程因子，建立了人牙龈成纤维细胞（hGFs）的诱导多能干细胞。本研究建立的hGF-iPSc表现出与人胚胎干细胞（ES）相似的形态和生长特性，并表达多能性细胞。碱性磷酸酶（AP）染色、胚状体（EB）形成、体外分化和核型分析进一步证实了hGF-iPSc的多能性。然后用成骨诱导培养基在机械、喷砂和酸蚀（SLA）处理的钛盘上培养hGF-iPSc，通过Alizrin Red S染色、扫描电镜（SEM）、流式细胞术和RT-PCR观察其不同表面类型的形态学和定量变化。结果：hGF-iPSc在成骨分化过程中出现了时间依赖性、表面依赖性的形态学变化和定量变化，在经sla处理的钛盘上培养的hGF-iPSc成骨基因表达量大于经机械处理的钛盘，提示hGF-iPSc的命运可能与hGF-iPSc首先粘附的表面特征有关。结论：牙干细胞诱导多能干细胞是一种极具潜力和实用价值的个性化再生牙科细胞来源，其不同表面类型诱导多能干细胞的形态学变化和成骨分化的定量变化可进一步应用于牙种植体的临床应用。
Objective: The aim of this study is to investigate the behaviour of iPSc derived from dental stem cells in terms of initial adhesion, differentiation potential on differently surface-treated titanium disc.Materials and methods: iPSc derived from human gingival fibroblasts (hGFs) were established using 4-reprogramming factors transduction with Sendai virus. The hGF-iPSc established in this study exhibited the morphology and growth properties similar to human embryonic stem (ES) cells and expressed pluripotency makers. Alkaline Phosphatase (AP) staining, Embryoid Body (EB) formation and in vitro differentiation and karyotyping further confirmed pluripotency of hGF-iPSc. Then, hGF-iPSc were cultured on machined-and Sandblasted and acid etched (SLA)-treated titanium discs with osteogenic induction medium and their morphological as well as quantitative changes according to different surface types were investigated using Alizrin Red S staining, Scanning electron microscopy (SEM), Flow cytometry and RT-PCR.Results: Time-dependent and surface-dependent morphological changes as well as quantitative change in osteogenic differentiation of hGF-iPSc were identified and osteogenic gene expression of hGF-iPSc cultured on SLA-treated titanium disc found to be greater than machined titanium disc, suggesting the fate of hGF-iPSc may be determined by the characteristics of surface to which hGF-iPSc first adhere.Conclusions: iPSc derived from dental stem cell can be one of the most promising and practical cell sources for personalized regenerative dentistry and their morphological change as well as quantitative change in osteogenic differentiation according to different surface types may be further utilized for future clinical application incorporated with dental implant.