Abutment Coating With Diamond-Like Carbon Films to Reduce Implant-Abutment Bacterial Leakage
Abutment Coating With Diamond-Like Carbon Films to Reduce Implant-Abutment Bacterial Leakage
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DOI：
10.1902/jop.2015.150362
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发表时间：
2016-02-02
影响因子：
4.3
通讯作者：
Nogueira, Lafayette, Jr.
中科院分区：
文献类型：
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作者：
Cardoso, Mayra;Sangalli, Jorgiana;Nogueira, Lafayette, Jr.
背景资料：评价类金刚石碳（DLC）膜对外六角形（EH）和内六角形（IH）设计的基台和牙种植体之间界面细菌渗漏的影响。方法：采用等离子体增强化学气相沉积法沉积DLC膜。种植体和基台组（每组n = 30，每组180个种植体）根据基台基底的连接设计和处理进行划分：1）无处理（对照）; 2）DLC膜沉积; 3）Ag-DLC膜沉积。在无菌条件下，将1 μ L粪肠球菌接种在种植体内，并拧紧基台。检测器械组的即刻外部污染，将其悬浮在含有无菌培养肉汤的试管中，并随访5天。肉汤的浑浊指示细菌泄漏。在该阶段结束时，移除基台，用纸尖收集种植体的内部内容物，并将其接种在皮氏培养皿中。孵育24小时后，评估其细菌活力和菌落形成单位计数。结果：EH种植体的细菌渗漏率为16.09%，IH种植体的细菌渗漏率为80.71%（P
Background: The influence of diamond-like carbon (DLC) films on bacterial leakage through the interface between abutments and dental implants of external hexagon (EH) and internal hexagon (IH) designs was evaluated.Methods: Film deposition was performed by plasma-enhanced chemical vapor deposition. Sets of implants and abutments (n = 30 per group, sets of 180 implants) were divided according to connection design and treatment of the abutment base: 1) no treatment (control); 2) DLC film deposition; and 3) Ag-DLC film deposition. Under sterile conditions, 1 mu L Enterococcus faecalis was inoculated inside the implants, and abutments were tightened. The sets were tested for immediate external contamination, suspended in test tubes containing sterile culture broth, and followed for 5 days. Turbidity of the broth indicated bacterial leakage. At the end of the period, the abutments were removed and the internal content of the implants was collected with paper points and plated in Petri dishes. After 24-hour incubation, they were assessed for bacterial viability and colony-forming unit counting. Bacterial leakage was analyzed by chi(2) and Fisher exact tests (alpha = 5%).Results: The percentage of bacterial leakage was 16.09% for EH implants and 80.71% for IH implants (P