Total erythrocyte destruction time: the new index for the hemolytic performance of rotary blood pumps.
Total erythrocyte destruction time: the new index for the hemolytic performance of rotary blood pumps.
复制标题
总红细胞破坏时间：旋转式血泵溶血性能的新指标。
DOI：
10.1111/j.1525-1594.1995.tb02383.x
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发表时间：
1995
影响因子：
2.4
通讯作者：
Y. Nosé
中科院分区：
文献类型：
--
作者：
T. Shimono;K. Makinouchi;Y. Nosé
到目前为止，溶血指数是评价血泵体外溶血性能的唯一参数。然而，这一指标并不能反映红细胞的亚致死状态。本文提出了一个新的概念，即红细胞的完全破坏，用以表示红细胞的损伤特征。使用BioMedicus泵BP80和Nikkio泵HMS12进行了五个配对的长期体外溶血试验。泵运行到血液颜色突然变为深红色，或者泵运行超过24小时。在某个时间点，除一例尼基索病例外，所有实验中的无血浆血红蛋白水平都突然大幅上升。在某个时间点上，大多数红细胞的破坏突然同时发生。我们将这种现象称为红细胞完全破坏，并将将血浆游离血红蛋白水平提高到总血红蛋白的50%所需的泵送时间定义为总破坏时间。虽然两种泵的归一化溶血指数无统计学差异，但BioMedicus泵的总破坏时间明显短于Nikkio泵。看来，亚致死性损伤对红细胞的累积导致了整个红细胞溶血阈值的降低，也导致了红细胞的突然完全破坏。
To date an index of hemolysis is the only parameter to evaluate in vitro the hemolytic properties of blood pumps. However, this index does not reflect the sublethal conditions of erythrocytes. In this paper, a new concept, the total destruction of erythrocytes, to indicate traumatic features on erythrocytes is proposed. Five paired long duration in vitro hemolysis tests were conducted using the BioMedicus pump; BP80, and the Nikkiso pump, HMS12. The pumps ran until either a sudden change in blood color to dark red was noted or the pumps ran for over 24 h. At a certain point, the plasma-free hemoglobin levels suddenly and dramatically increased in each experiment except one Nikkiso case. At a certain point in time, the destruction of most erythrocytes suddenly and simultaneously occurred. We call this phenomenon total destruction of erythrocytes and define the pumping duration required to raise the level of the plasma-free hemoglobin to 50% of the total hemoglobin as total destruction time. Although there is no statistical difference in the normalized index of hemolysis between the two pumps, the total destruction time of the BioMedicus pump is significantly shorter than that of the Nikkiso pump. It appears that an accumulation of sublethal damage on erythrocytes resulted in the decrease of the hemolytic threshold of the entire erythrocyte and also resulted in a sudden entire destruction of erythrocytes.