Avian paramyxoviruses and influenza viruses isolated from mallard ducks (Anas platyrhynchos) in New Zealand
Avian paramyxoviruses and influenza viruses isolated from mallard ducks (Anas platyrhynchos) in New Zealand
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DOI：
10.1007/s00705-002-0818-2
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发表时间：
2002-01-01
影响因子：
2.7
通讯作者：
Gould, AR
中科院分区：
文献类型：
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作者：
Stanislawek, WL;Wilks, CR;Gould, AR
采用病毒学和血清学方法对新西兰健康野鸭（Anas platyrhynchos）感染禽副粘病毒（APMV）和流感病毒（AIV）的状况进行了综合研究。从321份气管和泄殖腔拭子中分离的33种病毒的特征为：6种AIV（2种H5N2和4种H4N6），10种APMV-1和17种APMV-4。用核蛋白阻断ELISA法检测335份血清中AIV抗体，阳性109份（32.5%）。通过血凝抑制试验检测血清样品（315份）中的APMV-1、-2、-3、-4、-6、-7、-8、-9抗体。滴度大于或等于1/64的反应数量最多的是APMV-1（93.1%），其次是APMV-6（85.1%）、APMV-8（56%）、APMV-4（51.7%）、APMV-7（47%）、APMV-9（15.9%）、APMV-2（13.3%）和APMV-3（6.0%）。通过静脉内致病性指数（IVPI）和脑内致病性指数（ICPI）评估，来自本次和早期新西兰研究的所有AIV H5N2分离株和APMV-1分离株的致病性指数均较低，结果为0.00 - 0.16。部分基因组和抗原分析也与非致病性分离株一致。对10个APMV-1分离物的系统发育分析表明，9个分离物与最初在日本分离的参考APMV-1菌株D26/76以及在澳大利亚分离的Que/66菌株最相似。另一个分离物与从法国麻鸭中获得的病毒（MC 110/77）非常相似。
A comprehensive study using virological and serological approaches was carried out to determine the status of live healthy mallard ducks (Anas platyrhynchos) in New Zealand for infections with avian paramyxoviruses (APMV) and influenza viruses (AIV). Thirty-three viruses isolated from 321 tracheal and cloacal swabs were characterized as: 6 AIV (two H5N2 and four H4N6), 10 APMV-1 and 17 APMV-4. Of 335 sera samples tested for AIV antibodies, 109 (32.5%) sera were positive by nucleoprotein-blocking ELISA (NP-B-ELISA). Serum samples (315) were examined for antibody to APMV-1, -2, -3, -4, -6, -7, -8, -9 by the haemagglutination inhibition test. The largest number of reactions, with titres up to greater than or equal to 1/64, was to APMV-1 (93.1%), followed by APMV-6 (85.1%), APMV-8 (56%), APMV-4 (51.7%), APMV-7 (47%), APMV-9 (15.9%), APMV-2 (13.3%) and APMV-3 (6.0%). All of the H5N2 isolates of AIV and the APMV-1 isolates from this and earlier New Zealand studies had low pathogenicity indices assessed by the Intravenous Pathogenicity Index (IVPI) with the result 0.00 and Intracerebral Pathogenicity Index (ICPI) with results 0.00-0.16. Partial genomic and antigenic analyses were also consistent with the isolates being non-pathogenic. Phylogenetic analysis of the 10 APMV-1 isolates showed 9 to be most similar to the reference APMV-1 strain D26/76 originally isolated in Japan and also to the Que/66 strain, which was isolated in Australia. The other isolate was very similar to a virus (MC 110/77) obtained from a shelduck in France.