Targeted chimera delivery to ovarian cancer cells by heterogeneous gold magnetic nanoparticle
Targeted chimera delivery to ovarian cancer cells by heterogeneous gold magnetic nanoparticle
复制标题
通过异质金磁性纳米颗粒将嵌合体靶向递送至卵巢癌细胞
DOI：
10.1088/0957-4484/28/2/025101
复制
发表时间：
2017-01-13
期刊：
影响因子：
3.5
通讯作者：
Shi, Donglu
中科院分区：
文献类型：
--
作者：
Chen, Yao;Xu, Mengjiao;Shi, Donglu
小干扰RNA（siRNA）的有效递送至靶细胞在临床应用中仍然是一个重大挑战。在本研究中，我们开发了一种新的适配子-siRNA嵌合体的传输系统介导的阳离子Au-Fe 3 O 4纳米粒子（NPs）。将VEGF RNA适配子与Notch 3 siRNA构建的嵌合体通过静电作用与异质性Au-Fe 3 O 4纳米颗粒结合。与单独的嵌合体和Lipofectamine-siRNA复合物相比，所获得的复合物对Notch 3基因的沉默效率要高得多，并且提高了负载嵌合体的抗肿瘤效果。此外，Au-Fe 3 O 4纳米颗粒的嵌合体的有效递送可以逆转卵巢癌细胞对化疗药物顺铂的多药耐药（MDR），表明其在克服MDR的同时具有未来靶向癌症治疗的潜在能力。
Efficient delivery of small interfering RNAs (siRNAs) to the targeted cells has remained a significant challenge in clinical applications. In the present study, we developed a novel aptamer-siRNA chimera delivery system mediated by cationic Au-Fe3O4 nanoparticles (NPs). The chimera constructed by VEGF RNA aptamer and Notch3 siRNA was bonded with heterogeneous Au-Fe3O4 nanoparticles by electrostatic interaction. The obtained complex exhibited much higher silencing efficiency against Notch3 gene compared with chimera alone and lipofectamine-siRNA complex, and improved the antitumor effects of the loaded chimera. Moreover, the efficient delivery of the chimera by Au-Fe3O4 NPs could reverse multi-drug resistance (MDR) of ovarian cancer cells against the chemotherapeutic drug cisplatin, indicating its potential capability for future targeted cancer therapy while overcoming MDR.