The protective effect of cilostazol on isolated rabbit femoral arteries under conditions of ischemia and reperfusion: the role of the nitric oxide pathway.
The protective effect of cilostazol on isolated rabbit femoral arteries under conditions of ischemia and reperfusion: the role of the nitric oxide pathway.
复制标题
在缺血和再灌注条件下，西洛替唑对孤立的兔股动脉的保护作用：一氧化氮途径的作用。
DOI：
10.6061/clinics/2012(02)13
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发表时间：
2012
期刊：
影响因子：
--
通讯作者：
Joviliano EE
中科院分区：
文献类型：
--
作者：
Santos MR;Celotto AC;Capellini VK;Evora PR;Piccinato CE;Joviliano EE
下肢缺血/再灌注的临床意义需要进一步研究，以开发更有效的治疗替代方案。本研究观察了西洛他唑对兔股动脉局部缺血/再灌流条件下血管反应性和一氧化氮代谢产物的影响。采用肾下主动脉夹闭法造成缺血模型。实验动物随机分为7组：对照组90min、缺血再灌注90min/60min、对照组120min、缺血再灌注120min/90min、西洛他唑、西洛他唑缺血前120min/90min、缺血120min/西洛他唑/再灌注90min。获得了硝普钠、乙酰胆碱和钙离子载体A23187在离体股动脉的剂量-反应曲线。用化学发光法测定血浆和骨骼肌中亚硝酸盐和硝酸盐的水平。在缺血/再灌流120/90组，乙酰胆碱和A23187诱导的松弛作用减弱，西洛他唑治疗可部分阻止这种缺血/再灌流引起的内皮损伤。只有西洛他唑治疗增加了血浆亚硝酸盐和硝酸盐的水平。缺血/再灌注120/90组、西洛他唑/缺血/再灌注组和缺血/西洛他唑/再灌注组肌肉组织中亚硝酸盐和硝酸盐水平升高。后肢缺血/再灌流导致股动脉内皮依赖性松弛受损。此外，缺血前给予西洛他唑对缺血/再灌流条件下的内皮依赖性血管反应性有保护作用。
The clinical significance of ischemia/reperfusion of the lower extremities demands further investigation to enable the development of more effective therapeutic alternatives. This study investigated the changes in the vascular reactivity of the rabbit femoral artery and nitric oxide metabolites under partial ischemia/reperfusion conditions following cilostazol administration. Ischemia was induced using infrarenal aortic clamping. The animals were randomly divided into seven groups: Control 90 minutes, Ischemia/Reperfusion 90/60 minutes, Control 120 minutes, Ischemia/Reperfusion 120/90 minutes, Cilostazol, Cilostazol before Ischemia/Reperfusion 120/90 minutes, and Ischemia 120 minutes/Cilostazol/Reperfusion 90 minutes. Dose-response curves for sodium nitroprusside, acetylcholine, and the calcium ionophore A23187 were obtained in isolated femoral arteries. The levels of nitrites and nitrates in the plasma and skeletal muscle were determined using chemiluminescence. Acetylcholine- and A23187-induced relaxation was reduced in the Ischemia/Reperfusion 120/90 group, and treatment with cilostazol partially prevented this ischemia/reperfusion-induced endothelium impairment. Only cilostazol treatment increased plasma levels of nitrites and nitrates. An elevation in the levels of nitrites and nitrates was observed in muscle tissues in the Ischemia/Reperfusion 120/90, Cilostazol/Ischemia/Reperfusion, and Ischemia/Cilostazol/Reperfusion groups. Hind limb ischemia/reperfusion yielded an impaired endothelium-dependent relaxation of the femoral artery. Furthermore, cilostazol administration prior to ischemia exerted a protective effect on endothelium-dependent vascular reactivity under ischemia/reperfusion conditions.
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影响因子：
4.1
作者：
KAPLAN, RS;PEDERSEN, PL
通讯作者：
PEDERSEN, PL
影响因子：
3.4
作者：
Manickavasagam, Saraswathy;Ye, Yumei;Birnbaum, Yochai
通讯作者：
Birnbaum, Yochai
影响因子：
64.8
作者：
Dimmeler, S;Fleming, I;Zeiher, AM
通讯作者：
Zeiher, AM
DOI：
10.1111/j.1440-1681.2011.05550.x
发表时间：
2011-10-01
影响因子：
2.9
作者：
Bai, Yushan;Muqier;Minatoguchi, Shinya
通讯作者：
Minatoguchi, Shinya
DOI：
10.1152/ajpheart.1999.276.5.h1567
发表时间：
1999-05-01
影响因子：
4.8
作者：
Jones, SP;Girod, WG;Lefer, DJ
通讯作者：
Lefer, DJ