The dual specificity protein kinase CLK3 is abundantly expressed in mature mouse spermatozoa
The dual specificity protein kinase CLK3 is abundantly expressed in mature mouse spermatozoa
复制标题
DOI：
10.1006/excr.1999.4655
复制
发表时间：
1999-12-15
影响因子：
3.7
通讯作者：
Landreth, G
中科院分区：
文献类型：
--
作者：
Menegay, H;Moeslein, F;Landreth, G
CLK3 是双特异性蛋白激酶 LAMMER 家族的成员，在雄性小鼠的生殖系统中大量表达。具体来说，在睾丸和附睾的成熟精子中发现了高水平的 CLK3 蛋白表达。睾丸中的大部分 CLK3 蛋白是含有全长激酶的形式，仅观察到少量催化失活的 N 端截短的剪接变体蛋白产物。在成熟精子中，CLK3 定位于顶体和尾部。 CLK3 在顶体反应后从精子中排出并失活，可能是由于顶体反应期间精子释放的蛋白酶降解所致。 CLK 激酶家族此前已被证实参与 mRNA 剪接；然而，这些细胞中的 CLK3 蛋白大部分位于细胞质中，表明 CLK3 可能在细胞中具有其他作用。 (C) 1999 年学术出版社。
CLK3, a member of the LAMMER family of dual-specificity protein kinases, is abundantly expressed in the reproductive system of male mice. Specifically, high levels of CLK3 protein expression are found in mature spermatozoa in the testis and epididymis. The majority of the CLK3 protein in the testis is a full-length kinase-containing form, and only a small amount of a catalytically inactive N-terminally truncated splice variant protein product is observed. Within the mature spermatozoa CLK3 is localized to the acrosome and tail. CLK3 is expelled from the sperm following the acrosome reaction and inactivated, likely by degradation by the proteases released by the sperm during the acrosome reaction. The CLK family of kinases has previously been implicated in mRNA splicing; however, the bulk of the CLK3 protein in these cells is located in the cytoplasm, suggesting that CLK3 may have additional roles in the cell. (C) 1999 Academic Press.