HUVEC Tube-formation Assay to Evaluate the Impact of Natural Products on Angiogenesis
HUVEC Tube-formation Assay to Evaluate the Impact of Natural Products on Angiogenesis
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DOI：
10.3791/58591
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发表时间：
2019-06-01
影响因子：
1.2
通讯作者：
Colucci-D'Amato, Luca
中科院分区：
文献类型：
--
作者：
Gentile, Maria Teresa;Pastorino, Olga;Colucci-D'Amato, Luca
血管生成是一种包括不同过程的现象，例如内皮细胞增殖、分化和迁移，这些过程导致新血管的形成并涉及多种信号转导途径。在这里，我们证明管形成测定是一种简单的体外方法，用于评估天然产物对血管生成的影响并研究所涉及的分子机制。特别是，在芸香水提取物 (RGWE) 存在的情况下，内皮细胞不再能够形成细胞-细胞网络，并且 RGWE 对人脐静脉内皮细胞 (HUVEC) 管形成的影响被 MEK 的组成型激活所消除。
Angiogenesis is a phenomenon that includes different processes, such as endothelial cell proliferation, differentiation, and migration, that lead to the formation of new blood vessels and involve several signal transduction pathways. Here we show that the tube formation assay is a simple in vitro method to evaluate the impact of natural products on angiogenesis and to investigate the molecular mechanisms involved. In particular, in the presence of the water extract of Ruta graveolens (RGWE), endothelial cells are no longer able to form a cell-cell network and that the RGWE effects on human umbilical vein endothelial cell (HUVEC) tube formation is abolished by the constitutive activation of MEK.