Studies on the nitrogen metabolism of the ensilage process.
Studies on the nitrogen metabolism of the ensilage process.
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DOI：
10.1002/jsfa.2740070206
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发表时间：
1956-01-01
影响因子：
4.1
通讯作者：
Kemble, A. R.
中科院分区：
文献类型：
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作者：
Kemble, A. R.
以牛奶瓶为容器，通过添加厌氧细菌的悬浮液或乳酸杆菌的悬浮液沿着葡萄糖溶液，从年轻的多年生黑麦草（干物质中含氮4.71%）制成坏的和好的青贮饲料。在302 ℃下储存后。对于1和3天以及1、3、8和21周，通过先前描述的方法（Absts. 304，305，第25卷）。此外，年轻的蒂莫西从无菌生长室，青贮在试管中，16天后进行了检查。结果列表显示，在前3天氨基酸迅速上升;即使在良好的青贮饲料中，约三分之一的蛋白质在这个阶段被分解。后来，即使在pH值3.8有一个缓慢的稳定上升的可溶性N，使它有可能有一个青贮饲料的pH值，挥发性碱含量和气味良好的青贮饲料，虽然一半的原始蛋白质已被打破。发现的氨基酸的量，除了丙氨酸在坏的青贮饲料，是小于从蛋白质分解率计算。来自无菌草的青贮饲料的结果表明，过量的丙氨酸和蛋白质的快速初始分解是由于植物酶，而不是微生物。W. M.研究了在好的、坏的和无微生物的实验室“青贮饲料“中发生的氮分配的总体变化。这些结果使得能够区分在筒仓中同时发生的一些酶反应和细菌反应。M.P.
With quart milk bottles as containers, bad and good silage was made from young perennial ryegrass (4.71 per cent. N in the dry matter) by adding a suspension of anaerobic bacteria, or a suspension of lactobacilli along with glucose solution. After storage at 302 C. for 1 and 3 days and 1, 3, 8 and 21 weeks, total and soluble N, volatile base, total amide, glutamine, alpha-amino N, alcoholinsoluble peptide N and free amino acids were estimated by methods described earlier (Absts. 304, 305, Vol. 25). Also, young timothy from a sterile growth chamber, ensiled in a test tube, was examined after 16 days. The results are tabulated and show a rapid rise of amino acids in the first 3 days; even in the good silage, about one-third of the protein was broken down at this stage. Later, even at pH 3.8 there was a slow steady rise of soluble N, so that it was possible to have a silage with the pH, volatile base content and odour of good silage although half the original protein had been broken down. The amounts of amino acids found, except for alanine in the bad silage, were less than those calculated from the rate of protein breakdown. Results for the silage from sterile grass indicated that the excess alanine, and the rapid initial breakdown of protein, are due to the plant enzymes, not to microorganisms.-W. M. Deans.The overall changes in N distribution occurring in good, bad and micro-organism-free laboratory " silage " were investigated. The results enabled a distinction to be drawn between some of the enzymic and bacterial reactions which occur simultaneously in the silo.-M.P.