Sensitive electrochemical sensing platform for microRNAs detection based on shortened multi-walled carbon nanotubes with high-loaded thionin
Sensitive electrochemical sensing platform for microRNAs detection based on shortened multi-walled carbon nanotubes with high-loaded thionin
复制标题
基于高负载硫堇短多壁碳纳米管的灵敏电化学传感平台，用于 microRNA 检测
DOI：
10.1016/j.bios.2018.05.055
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发表时间：
2018-10-15
影响因子：
12.6
通讯作者：
Huang, Haowen
中科院分区：
文献类型：
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作者：
Deng, Keqin;Liu, Xinyan;Huang, Haowen
比较了短多壁碳纳米管（S-MWCNTs）和酸化多壁碳纳米管（A-MWCNTs）上硫堇（Thi）的负载能力。设计了两个 DNA 探针片段，用于与 microRNA (miRNA) 模型分析物 microRNA-21 (miR-21) 杂交。 DNA 探针 1 (P1) 组装在金纳米颗粒 (AuNPs) 修饰电极上。 MiR-21 被预固定的 P1 捕获。通过在 S-MWCNT 上负载大量 Thi 并与共价键合的探针 2 (P2) 合成信号纳米探针。由于多壁碳纳米管的有效表面积大、多壁碳纳米管的快速电子穿梭、S-多壁碳纳米管上的高负载Thi以及AuNPs增加的电导率，信号探针与miR-21杂交后，在电极上产生了放大的电流响应。增加的电化学电流使我们能够定量检测 miR-21。该策略避免了昂贵的生物试剂和标记的目标/检测 DNA 或 miRNA。操作复杂性和检测成本也降低了。
The loading capacity of thionin (Thi) on shortened multi-walled carbon nanotubes (S-MWCNTs) and acidified multi-walled carbon nanotubes (A-MWCNTs) was compared. Two DNA probe fragments were designed for hybridization with microRNA-21 (miR-21), the microRNAs (miRNAs) model analyte. DNA probe 1 (P1) was assembled on Au nanoparticles (AuNPs) modified electrode. MiR-21 was captured by the pre-immobilized P1. A signal nanoprobe was synthesized by loading large amount of Thi on S-MWCNTs with covalently bonded probe 2 (P2). Owing to the large effective surface area of MWCNTs, fast electron shuttle of MWCNTs, high-loaded Thi on S-MWCNTs, and the increased conductivity from AuNPs, after signal probe hybridized with miR-21, it gave rise to a magnified current response on electrode. The increased electrochemical current enabled us to quantitatively detect miR-21. Expensive bioreagents and labeled target/detection DNA or miRNAs were avoided in this strategy. The operation complexity and assay cost were also reduced.