The type III secretion system effector network hypothesis
The type III secretion system effector network hypothesis
复制标题
III型分泌系统效应网络假说
DOI：
10.1016/j.tim.2021.10.007
复制
发表时间：
2022
影响因子：
15.9
通讯作者：
Sanchez-Garrido J
中科院分区：
文献类型：
--
作者：
Sanchez-Garrido J
III型分泌系统（T3SS）效应子是支撑许多临床上重要的革兰氏阴性病原体的感染策略的关键毒力因子，所述革兰氏阴性病原体包括肠道沙门氏菌（Salmonella enterica）、志贺氏菌（Shigellaspp.）肠致病性和肠破坏性大肠杆菌及其鼠等效物，啮齿类柠檬酸杆菌。效应物靶向的细胞过程或蛋白质可以是多种病原体共有的或病原体特异性的。理解T3SS介导的发病机制的主要方法是一次确定一个效应子的贡献，目的是将个体功能拼凑在一起并揭示感染机制。然而，与这种流行的方法相反，同时删除多个效应子揭示了它们在体内起着相互连接的网络的作用，揭示了效应子的相互依赖性和上下文依赖性效应子的重要性。T3SS生物学的这种范式转变是这篇观点文章的核心。
Type III secretion system (T3SS) effectors are key virulence factors that underpin the infection strategy of many clinically important Gram-negative pathogens, includingSalmonella enterica,Shigellaspp., enteropathogenic and enterohemorrhagicEscherichia coliand their murine equivalent,Citrobacter rodentium. The cellular processes or proteins targeted by the effectors can be common to multiple pathogens or pathogen-specific. The main approach to understanding T3SS-mediated pathogenesis has been to determine the contribution of one effector at a time, with the aim of piecing together individual functions and unveiling infection mechanisms. However, in contrast to this prevailing approach, simultaneous deletion of multiple effectors revealed that they function as an interconnected networkin vivo, uncovering effector codependency and context-dependent effector essentiality. This paradigm shift in T3SS biology is at the heart of this opinion article.