Differential expression of cytokines, chemokines and chemokine receptors in patients with coronary artery disease
Differential expression of cytokines, chemokines and chemokine receptors in patients with coronary artery disease
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DOI：
10.1016/j.ijcard.2008.04.009
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发表时间：
2009-07-24
影响因子：
3.5
通讯作者：
Souza Lima Blotta, Maria Heloisa
中科院分区：
文献类型：
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作者：
Dias de Oliveira, Romulo Tadeu;Mamoni, Ronei Luciano;Souza Lima Blotta, Maria Heloisa
单核细胞/巨噬细胞和淋巴细胞通过产生炎症和抗炎细胞因子在动脉粥样硬化的发病机制中发挥关键作用。我们在体外评估了 CCL2、CXCL8、CXCL9、CXCL10、IFN-γ 和 IL-10 的 mRNA 表达和蛋白质产生，以及在存在或不存在氧化 LDL (oxLDL) 的情况下，冠状动脉疾病 (CAD) 患者和健康对照的外周血单核细胞 (PBMC) 中 CCR2 和 CXCR3 受体的表达。 CAD患者表现出较高的CCL2、CXCL8、CXCL9、CXCL10和IFN-γ mRNA的组成型表达，并且在用oxLDL刺激后，CCL2和CXCL8 mRNA的表达高于对照组。我们还在 CAD 患者的 oxLDL 刺激的 PBMC 上清液中检测到 CCL2 和 CXCL8 水平高于对照的相应上清液。接受oxLDL刺激后，CAD患者的组成型CCR2(+)和CXCR3(+)细胞比例较高。在 CAD 患者中，稳定型心绞痛 (SA) 组和不稳定型心绞痛 (UA) 组之间的主要区别是后者的 IL-10 mRNA 产生较低。总而言之，我们的数据表明，来自 CAD 患者的 PBMC 能够产生较高浓度的趋化因子和细胞因子，参与调节单核细胞和淋巴细胞的迁移和在动脉粥样硬化病变中的保留。 (C) 2008 Elsevier Ireland Ltd. 保留所有权利。
Monocytes/macrophages and lymphocytes have a key role in the pathogenesis of atherosclerosis through the production of inflammatory and anti-inflammatory cytokines. We evaluated mRNA expression and protein production of CCL2, CXCL8, CXCL9, CXCL10, IFN-gamma and IL-10 in vitro as well as the expression of the CCR2 and CXCR3 receptors in peripheral blood mononuclear cells (PBMCs) of patients with coronary artery disease (CAD) and healthy controls in the presence or absence of oxidized LDL (oxLDL). Patients with CAD showed higher constitutive expression of CCL2, CXCL8, CXCL9, CXCL10 and IFN-gamma mRNA and, after stimulation with oxLDL, higher expression of CCL2 and CXCL8 mRNA than the control group. We also detected higher levels of CCL2 and CXCL8 in supernatants of oxLDL-stimulated PBMCs from CAD patients than in corresponding supernatants from controls. Patients with CAD had a higher percentage of constitutive CCR2(+) and CXCR3(+) cells after stimulation with oxLDL. Among CAD patients, the main differences between the stable (SA) and unstable angina (UA) groups were lower IL-10 mRNA production in the latter group. Altogether, our data suggest that PBMCs from CAD patients are able to produce higher concentrations of chemokines and cytokines involved in the regulation of monocyte and lymphocyte migration and retention in atherosclerotic lesions. (C) 2008 Elsevier Ireland Ltd. All rights reserved.