Engineering tissues with a perfusable vessel-like network using endothelialized alginate hydrogel fiber and spheroid-enclosing microcapsules.
Engineering tissues with a perfusable vessel-like network using endothelialized alginate hydrogel fiber and spheroid-enclosing microcapsules.
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DOI：
10.1016/j.heliyon.2016.e00067
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发表时间：
2016-02
期刊：
影响因子：
4
通讯作者：
Taya M
中科院分区：
文献类型：
--
作者：
Liu Y;Sakai S;Taya M
在体外构建致密三维组织的过程中，构建内部可灌注血管网的技术是一个具有挑战性的问题。在这里，我们报告了一种实现它的方法。我们组装了小组织（直径约200 μm）封闭的水凝胶微胶囊和单一的水凝胶纤维，两者都覆盖着胶原凝胶中的人血管内皮细胞。微胶囊和纤维由海藻酸盐和明胶衍生物制成，并具有细胞粘附表面。水凝胶构建体上的内皮细胞发芽并自发形成胶原凝胶中将水凝胶构建体彼此连接的网络。通过将含有直径约3 μm的示踪颗粒的溶液引入由藻酸盐水凝胶纤维模板化的管腔中，证实了在由藻酸盐裂解酶降解基于藻酸盐的水凝胶构建体之后形成可灌注的血管网络样结构。引入的溶液流入自发形成的毛细血管分支，并绕过单个球形组织。
Development of the technique for constructing an internal perfusable vascular network is a challenging issue in fabrication of dense three-dimensional tissues in vitro. Here, we report a method for realizing it. We assembled small tissue (about 200 μm in diameter)-enclosing hydrogel microcapsules and a single hydrogel fiber, both covered with human vascular endothelial cells in a collagen gel. The microcapsules and fiber were made from alginate and gelatin derivatives, and had cell adhesive surfaces. The endothelial cells on the hydrogel constructs sprouted and spontaneously formed a network connecting the hydrogel constructs with each other in the collagen gel. Perfusable vascular network-like structure formation after degrading the alginate-based hydrogel constructs by alginate lyase was confirmed by introducing solution containing tracer particles of about 3 μm in diameter into the lumen templated by the alginate hydrogel fiber. The introduced solution flowed into the spontaneously formed capillary branches and passed around the individual spherical tissues.