Tetrahymena promotes interactive transfer of carbapenemase gene encoded in plasmid between fecal Escherichia coli and environmental Aeromonas caviae
Tetrahymena promotes interactive transfer of carbapenemase gene encoded in plasmid between fecal Escherichia coli and environmental Aeromonas caviae
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DOI：
10.1111/1348-0421.12656
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发表时间：
2018-11
影响因子：
2.6
通讯作者：
Mizue Matsushita;Torahiko Okubo;Takaki Hasegawa;J. Matsuo;Takanori Watanabe;S. Iwasaki;T. Fukumoto;Kasumi Hayasaka;Kozi Akizawa;Chikara Shimizu;Hiroyuki Yamaguchi
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文献类型：
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作者：
Mizue Matsushita;Torahiko Okubo;Takaki Hasegawa;J. Matsuo;Takanori Watanabe;S. Iwasaki;T. Fukumoto;Kasumi Hayasaka;Kozi Akizawa;Chikara Shimizu;Hiroyuki Yamaguchi
四膜虫能促进质粒在大肠杆菌之间或从大肠杆菌中转移。大肠杆菌通过囊泡积累转化为肠道沙门氏菌。在这项研究中，纤毛虫是否促进编码blaIMP-1的质粒在粪便E.大肠杆菌和环境嗜水气单胞菌进行了研究。将两种细菌与或不与纤毛虫混合，并在30°C下孵育过夜。使用含头孢他啶（CAZ）的琼脂平板通过菌落计数估计质粒获得性细菌的频率，然后测定最小抑菌浓度（MIC）。含有纤毛虫的培养物在E.大肠杆菌和气单胞菌，频率为10−4至10−5。所有质粒获得性细菌均显示出对CAZ的MIC>128 μg/mL，并且通过blaIMP-1基因的PCR扩增证实了质粒转移。荧光观察表明，两种细菌聚集在同一囊泡和transwell隔离显着降低转移频率。虽然纤毛虫优先摄取E. coli而不是A.然而，这两种细菌共定位于纤毛虫的相同囊泡中，表明它们的相遇与基因转移有关。因此，纤毛虫相互促进质粒在E. coli和A.开玩笑。本研究的结果将有助于控制多重抗生素耐药菌的传播。
Tetrahymena can facilitate plasmid transfer among Escherichia coli or from E. coli to Salmonella Enteritidis via vesicle accumulation. In this study, whether ciliates promote the interactive transfer of plasmids encoding blaIMP‐1 between fecal E. coli and environmental Aeromonas caviae was investigated. Both bacteria were mixed with or without ciliates and incubated overnight at 30°C. The frequency of plasmid‐acquired bacteria was estimated by colony counts using an agar plate containing ceftazidim (CAZ) followed by determination of the minimum inhibitory concentration (MIC). Cultures containing ciliates interactively transferred the plasmid between E. coli and Aeromonas with a frequency of 10−4 to 10−5. All plasmid‐acquired bacteria showed a MIC against CAZ of >128 μg/mL and the plasmid transfer was confirmed by PCR amplification of the blaIMP‐1 gene. Fluorescent observation showed that both bacteria accumulated in the same vesicle and that transwell sequestering significantly decreased the transfer frequency. Although ciliates preferentially ingested E. coli rather than A. caviae, both bacteria were co‐localized into the same vesicles of ciliates, indicating that their meeting is associated with the gene transfer. Thus, ciliates interactively promote plasmid transfer between E. coli and A. caviae. The results of this study will facilitate control of the spread of multiple‐antibiotic resistant bacteria.