Fine mapping of a Phytophthora-resistance locus RpsGZ in soybean using genotyping-by-sequencing
Fine mapping of a Phytophthora-resistance locus RpsGZ in soybean using genotyping-by-sequencing
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DOI：
10.1186/s12864-020-6668-z
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发表时间：
2020-04-03
期刊：
影响因子：
4.4
通讯作者：
Nian, Hai
中科院分区：
文献类型：
--
作者：
Jiang, Bingzhi;Cheng, Yanbo;Nian, Hai
大豆疫霉（Phytophthora sojae， P. sojae）引起的根腐病（Phytophthora root rot， PRR）是世界范围内制约大豆生产的最严重病害之一。鉴定抗病基因并将其纳入优良品种是预防大豆病害的有效途径。利用抗性品种贵枣1与易感品种BRSMG68杂交的228个F-8:11重组自交系的有价值定位群体，以及该群体中相邻bin标记间平均距离为0.81 cM的高密度遗传连锁图谱，对候选基因进行了定位和探索。结果发现，龟杂1的PRR抗性受一个孟德尔位点控制，并在参比williams 82基因组3号染色体上精细定位到一个367.371 kb的基因组区域，包含19个基因，其中7个为抗病(R)样基因。对候选基因的实时荧光定量PCR分析显示，Glyma.03 g05300可能与PRR抗性有关。结论Rps基因座的精细定位将为大豆抗性基因的克隆和转移以及通过标记辅助选择选育大豆抗性品种奠定基础。
Background Phytophthora root rot (PRR) caused by Phytophthora sojae (P. sojae) is one of the most serious limitations to soybean production worldwide. The identification of resistance gene(s) and their incorporation into elite varieties is an effective approach for breeding to prevent soybean from being harmed by this disease. A valuable mapping population of 228 F-8:11 recombinant inbred lines (RILs) derived from a cross of the resistant cultivar Guizao1 and the susceptible cultivar BRSMG68 and a high-density genetic linkage map with an average distance of 0.81 centimorgans (cM) between adjacent bin markers in this population were used to map and explore candidate gene(s). Results PRR resistance in Guizao1 was found to be controlled by a single Mendelian locus and was finely mapped to a 367.371-kb genomic region on chromosome 3 harbouring 19 genes, including 7 disease resistance (R)-like genes, in the reference Willliams 82 genome. Quantitative real-time PCR assays of possible candidate genes revealed that Glyma.03 g05300 was likely involved in PRR resistance. Conclusions These findings from the fine mapping of a novel Rps locus will serve as a basis for the cloning and transfer of resistance genes in soybean and the breeding of P. sojae-resistant soybean cultivars through marker-assisted selection.