Canonical Wnt activity regulates trunk neural crest delamination linking BMP/noggin signaling with G1/S transition
Canonical Wnt activity regulates trunk neural crest delamination linking BMP/noggin signaling with G1/S transition
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DOI：
10.1242/dev.01424
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发表时间：
2004-11-01
期刊：
影响因子：
4.6
通讯作者：
Kalcheim, C
中科院分区：
文献类型：
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作者：
Burstyn-Cohen, T;Stanleigh, J;Kalcheim, C
迁移前神经脊细胞的分层依赖于骨形态发生蛋白/noggin之间的平衡和成功的G1/S转变。在这里，我们报告了骨形态发生蛋白通过典型的Wnt信号调节G1/S的转变和随后的波峰脱层。Noggin过表达抑制G1/S的转变，阻断G1/S阻止BMP诱导的分层；此外，WNT1的转录受到BMP和发育中的体节的刺激，从而抑制noggin的产生。干扰P-catenin和Lef/Tcf可抑制Gi/S转换、神经脊分层和多种骨形态发生蛋白依赖基因的转录，其中包括Cad6B、Pax3和Msx1，但不能抑制Slug、Sox9和FoxD3的转录。因此，我们认为，发育中的体节抑制了背管中noggin的转录，导致BMP的激活，从而产生WNTL。规范的Wnt信号反过来刺激G1/S转换和神经脊细胞运动的产生，而不依赖于它在早期神经脊规范中所建议的作用。
Delamination of premigratory neural crest cells depends on a balance between BMP/noggin and on successful G1/S transition. Here, we report that BMP regulates G1/S transition and consequent crest dellamination through canonical Wnt signaling. Noggin overexpression inhibits G1/S transition and blocking G1/S abrogates BMP-induced delamination; moreover, transcription of Wnt1 is stimulated by BMP and by the developing somites, which concomitantly inhibit noggin production. Interfering with P-catenin and LEF/TCF inhibits GI/S transition, neural crest delamination and transcription of various BMP- dependent genes, which include Cad6B, Pax3 and Msx1, but not that of Slug, Sox9 or FoxD3. Hence, we propose that developing somites inhibit noggin transcription in the dorsal tube, resulting in activation of BMP and consequent Wntl production. Canonical Wnt signaling in turn stimulates G1/S transition and generation of neural crest cell motility independently of its proposed role in earlier neural crest specification.