Pediatric acquired immunodeficiency syndrome: demonstration of B lymphocyte defects in vitro.
Pediatric acquired immunodeficiency syndrome: demonstration of B lymphocyte defects in vitro.
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儿科获得性免疫缺陷综合征：体外 B 淋巴细胞缺陷的证明。
DOI：
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发表时间：
1986
期刊：
影响因子：
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通讯作者：
R. Pahwa
中科院分区：
文献类型：
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作者：
S. Pahwa;S. Fikrig;R. Ménez;R. Pahwa
儿童获得性免疫缺陷综合征（艾滋病）的特点是反复的细菌感染，这是抗体缺乏性疾病的一个常见特征。本研究旨在调查15名6个月至6岁艾滋病或艾滋病相关复合体（ARC）儿童的B淋巴细胞功能。新分离的外周血B细胞自发分泌免疫球蛋白，以及淋巴细胞在多克隆和抗原刺激后产生的免疫球蛋白和抗体分泌细胞，在溶血斑块试验中被量化。尽管自发免疫球蛋白分泌过多，但B细胞在体外刺激后引起的反应在这些儿童中被抑制。对t依赖性和t非依赖性刺激的反应受到影响。免疫调节性T细胞和固有B细胞功能的研究表明，前体B细胞缺陷和免疫调节异常导致B细胞分化缺陷。这些发现表明，B淋巴细胞功能障碍是临床表现为艾滋病或艾滋病相关复合体的儿童HTLV III感染的一个整体特征。
Acquired immunodeficiency syndrome (AIDS) in childhood is characterized by recurrent bacterial infections, a feature common in antibody deficiency disorders. The present study was aimed at investigating B lymphocyte function in 15 children aged 6 months to 6 years with AIDS or AIDS-related complex (ARC). Spontaneous secretion of immunoglobulins by freshly isolated peripheral blood B cells and the generation of immunoglobulin and antibody-secreting cells in lymphocyte cultures after polyclonal and antigenic stimulation were quantified in hemolytic plaque assays. Despite excessive spontaneous immunoglobulin secretion, responses elicited by B cells after in vitro stimulation were depressed in these children. Responses to T-dependent as well as to T-independent stimuli were affected. Studies of immunoregulatory T cells and intrinsic B cell function suggested that deficient precursor B cells and abnormal immunoregulation contributed to the defects in B cell differentiation. These findings indicate that B lymphocyte dysfunction is an integral feature of HTLV III infection in children who clinically present as either AIDS or AIDS-related complex.