Direct Observations of Graphene Dispersed in Solution by Twilight Fluorescence Microscopy
Direct Observations of Graphene Dispersed in Solution by Twilight Fluorescence Microscopy
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DOI：
10.1021/acs.jpclett.7b00739
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发表时间：
2017-06-01
影响因子：
5.7
通讯作者：
Sano, Masahito
中科院分区：
文献类型：
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作者：
Matsuno, Yutaka;Sato, Yu-uya;Sano, Masahito
利用新开发的暮光荧光（TwiF）显微镜直接观察了溶液中的石墨烯和氧化石墨烯（GO）。将纳米碳分散体与高度浓缩的荧光染料溶液混合，放置在底部有观察镜的细胞中。TwiF显微镜利用两种光学过程提供微弱照明的背光，对漂浮在玻璃表面几百分钟内的纳米碳材料进行成像，并通过吸收和能量转移过程将氧化石墨烯可视化为黑暗图像，或者通过荧光团化学和自身荧光交替显示为明亮图像。从亚微米到亚毫米宽度的单个石墨烯和氧化石墨烯薄片在不同波长下清晰成像，根据所使用的染料进行选择。石墨烯可以与氧化石墨烯在同一溶液中共存。部分透明显示分层和网络结构。实现了对翻滚流运动的实时识别。在现场考察了溶剂的变化对玻璃表面粘附过程的影响。
Graphene and graphene oxide (GO) in solution were directly observed by a newly developed twilight fluorescence (TwiF) microscopy. A nanocarbon dispersion was mixed with a highly concentrated fluorescent dye solution and placed in a cell with a viewing glass at the bottom. TwiF microscopy images the nanocarbon material floating within a few hundred pm of the glass surface by utilizing two optical processes to provide a faintly illuminating backlight and visualizes GO as either a dark image by absorption and energy transfer processes or a bright image by alternation of fluorophore chemistry and autofluorescence. Individual graphene and GO sheets ranging from submicron to submillimeter widths were clearly imaged at different wavelengths, which were selectable based on the dye used. Graphene could be differentiated from GO coexisting in the same solution. Partial transparency revealed layering and network structures. Motions in tumbling flow were recognized in real time. An effect of changing the solvent and the process of adhesion on the glass surface were followed in situ.