Effects of oats on plasma cholesterol and lipoproteins in C57BL/6 mice are substrain specific
Effects of oats on plasma cholesterol and lipoproteins in C57BL/6 mice are substrain specific
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DOI：
10.1017/s000711450999211x
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发表时间：
2010-02-28
影响因子：
3.6
通讯作者：
Hellstrand, Per
中科院分区：
文献类型：
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作者：
Andersson, Kristina E.;Immerstrand, Tina;Hellstrand, Per
燕麦的降胆固醇作用已在动物和人类受试者中得到证实。然而，需要进一步研究含燕麦饮食的关键特性，以优化其使用。小鼠模型将是一个有价值的工具，但迄今为止很少有这样的研究发表。我们研究了燕麦麸皮对C57 BL/6小鼠两个亚系的血浆胆固醇和脂蛋白的影响。通过添加0.8%胆固醇和0.1%胆酸使西方饮食具有致动脉粥样硬化性。致动脉粥样硬化饮食4周后，血浆总胆固醇从1.86-2-53 mmol/l增加至3.77-4-40 mmol/l。在C57 BL/6 NCrl小鼠中，包含27%和40%的燕麦麸皮分别使血浆总胆固醇降低19%和24%，减少了从HDL到LDL + VLDL的转变，并导致粪便胆固醇排泄增加。燕麦麸对炎症标志物纤维蛋白原、血清淀粉样蛋白A或TNF-α的血浆水平没有影响。与C57 BL/6 NCrl小鼠中的发现相反，在喂食4周后，燕麦麸（27或40%）对C57 BL/6 JBomTac小鼠的血浆胆固醇没有持续影响。因此，C57 BL/6 NCrl小鼠喂养的致动脉粥样硬化饮食是一个很好的模型，燕麦的生理效应的研究，而来自C57 BL/6 J，提出了一个不同的饲养环境和可能具有功能的遗传差异，从C57 BL/6 N，是相当少的燕麦响应。目前的发现，两个亚系的小鼠对燕麦的反应不同，这具有实用价值，但也有助于阐明燕麦降低胆固醇作用的机制。
Cholesterol-lowering effects of oats have been demonstrated in both animals and human subjects. However, the crucial properties of oat-containing diets that determine their health effects need to be further investigated to optimise their use. A mouse model would be a valuable tool, but few such studies have been published to date. We investigated the effects of oat bran on plasma cholesterol and lipoproteins in two substrains of C57BL/6 mice. Western diet was made atherogenic by the addition of 0.8 % cholesterol and 0.1 % cholic acid. After 4 weeks on atherogenic diet, total plasma cholesterol had increased from 1.86-2-53 to 3.77-4-40 mmol/l. In C57BL/6NCrl mice, inclusion of 27 and 40% oat bran reduced total plasma cholesterol by 19 and 24 %, respectively, reduced the shift from HDL to LDL + VLDL and caused increased faecal cholesterol excretion. There was no effect of oat bran on plasma levels of the inflammatory markers fibrinogen, serum amyloid A or TNF-alpha. Contrary to findings in C57BL/6NCrl mice, there was no sustained effect of oat bran (27 or 40 %) on plasma cholesterol in C57BL/6JBomTac mice after 4 weeks of feeding. Thus, C57BL/6NCrl mice fed an atherogenic diet are a good model for Studies of physiological effects of oats, whereas a substrain derived from C57BL/6J, raised in a different breeding environment and likely possessing functional genetic differences from C57BL/6N, is considerably less responsive to oats. The present finding that two substrains of mice respond differently to oats is of practical value, but can also help to elucidate mechanisms of the cholesterol-lowering effect of oats.