DEVELOPMENT OF 3T3-LIKE LINES FROM BALB/C MOUSE EMBRYO CULTURES - TRANSFORMATION SUSCEPTIBILITY TO SV40
DEVELOPMENT OF 3T3-LIKE LINES FROM BALB/C MOUSE EMBRYO CULTURES - TRANSFORMATION SUSCEPTIBILITY TO SV40
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DOI：
10.1002/jcp.1040720208
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发表时间：
1968-01-01
影响因子：
5.6
通讯作者：
TODARO, GJ
中科院分区：
文献类型：
--
作者：
AARONSON, SA;TODARO, GJ
在严格的移植计划下进行的BALB/c小鼠胚胎培养，最大限度地减少了细胞与细胞的接触，并进化成具有与3T3非常相似的特性的永久系。从相同的胚胎培养中，细胞与细胞接触广泛的移植计划将导致形成多个细胞层的细胞系(BALB/3T12)，其饱和密度最高可达BALB/3T3系的25倍。Balb/3T3与3T3的形态相似，能在高稀释、低饱和密度下生长，对SV40病毒的转化具有较高的敏感性。继续保持高细胞密度的Balb/3T12细胞系在克隆效率上几乎没有提高。感染SV40产生的转化子可以根据它们在低细胞密度下形成集落的能力进行选择。选择合适的选择系统，SV40T抗原在Balb/3T3和Balb/3T12中的诱导和转化似乎具有可比性。
Balb/c mouse embryo cultures that are carried under a rigid transfer schedule that minimizes cell‐cell contact evolve into permanent lines that possess properties very similar to 3T3. From the same embryo cultures, a transfer schedule where cell‐cell contact is extensive leads to lines (Balb/3T12) that form multiple cell layers and have saturation densities up to 25‐fold higher than the Balb/3T3 lines. All the lines are hypotetraploid.Comparison of the Balb/3T3 lines with 3T3 reveals similar morphology, ability to grow at high dilution, low saturation density, and high susceptibility to transformation by SV40 virus. The Balb/3T12 lines, which are continually maintained at high cell density, show little improvement in cloning efficiency. Infection with SV40 produces transformants that can be selected by their ability to form colonies at low cell densities. Using the appropriate selective system for each line, it appears that SV40 T antigen induction and transformation in Balb/3T3 and Balb/3T12 are comparable.